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Yeast Research Tools

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G-Biosciences offers a large selection of products specifically designed for yeast research, whether working with DNA or looking at proteins. 

Yeast DNA Related Products

Three kits are offered for the extraction of genomic DNA from yeast. OmniPrep™ for Yeast allows for the purification of highly pure genomic DNA, XIT™ genomic DNA from yeast uses lytic digestion and protein precipitation to release high quality DNA and Yeast Geno-DNA-Template™ is ideal for rapid extraction yeast DNA for use as a template in PCR. 

For rapid single step yeast transformation in under a minute our Fast Yeast Transformation Kit is highly efficient and our EZ Yeast™ Plasmid Prep is designed for the rapid isolation of 2μ plasmids from yeast patches or yeast grown in small liquid cultures. Longlife Zymolyase®, a highly stable and ready-to-use yeast lytic enzyme is also available. 

Yeast Protein Related Products

For the extraction of proteins from yeast , two lysis buffer systems are offered to extract active, soluble proteins, PopLysis™ Yeast Lysis and Extraction and Yeast PE LB™. For the extraction of the total yeast proteome the chaotropic FOCUS™ Yeast Proteome is recommended. To preserve your protein of interest, a yeast specific protease inhibitor cocktail should be used, Yeast/ Fungal ™​ProteaseArrest.  Saccharomyces cerevisiae GenLysate™ is a yeast cell lysate of total proteins, extracted from Saccharomyces cerevisiae and is ready to use for SDS-PAGE and Western blotting purposes. Several kits are offered for the extraction and purification of 6X His or GST tagged recombinant proteins from yeast. 

The EZ Yeast™ Plasmid Prep kit is designed for the rapid isolation of 2μ plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and our LongLife™ Zymolyase®, a highly ..
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Fast Yeast Transformation™ kit is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformatio..
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Specifically designed for yeast research and supplied with yeast specific reagents. Extracts and solubilizes nearly all of the proteins from yeast, including membrane and soluble proteins.  Extraction is based on gentle lysis of yeast cells with a yeast lytic enzyme preparation, LongLife™..
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HOOK™ 6X His Protein Purification kit allows for the purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of ..
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HOOK™ 6X His Protein Spin Purification kit allows for the rapid purification of soluble, 6X His tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and a..
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HOOK™ GST Protein Purification kit allows for the purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activity of the pr..
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HOOK™ GST Protein Spin Purification kit allows for the rapid purification of soluble, GST tagged protein from yeast cultures. The yeast are first lysed with Yeast PE LB™ and LongLife™ Zymolyase® to release total soluble protein, whilst maintaining the structure and activit..
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Enzymes regularly used in laboratory applications often require preparation of fresh solution before each use. Making fresh enzyme solution for each application is time consuming and wasteful. G-Biosciences offers a wide variety of fresh, ready-to-use enzyme preparations.   Our high perf..
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Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  A selection of genomic isolation kits are offered that purify high quality genomic DNA  from a wide variety of sources and for a wide a..
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The XIT™ Genomic DNA from Yeast kit is designed for the isolation of genomic DNA from overnight yeast cultures.   The kit uses three main steps (see figure) that are rapid & efficient enzymatic digestion of cell walls, spheroplast lysis and complete protein precipitation, follo..
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The Yeast Geno-DNA-Template™ extraction kit isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitati..
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Yeast PE LB™ is useful for extraction of soluble proteins from yeast cells. Yeast PE LB™ is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells. This kit is provided with an optional protocol to make spheroplas..
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