PopLysis™ Protein Extraction Systems
PopLysis™ protein extraction systems utilize a cocktail of membrane solubilizing detergents and are optimized to rapidly solubilize and “pop open” membrane structures, allowing cellular proteins to spill out into lysis solution. The cellular proteins are stable in PopLysis™ buffer and suitable for downstream applications such as enzyme assays, chromatography studies, ELISA, Western blot, protein folding studies, and gel electrophoresis. These systems are also suitable for high throughput applications such as screening recombinant proteins. PopLysis™ buffers contain amine-free buffering agents making them suitable for applications that require proteins in amine free buffers. PopLysis™ buffer systems are optimized kits for lysis and extraction of proteins from bacteria, yeast, neurons, mammalian cells, mammalian tissue, and insect cells.