DNA Clean Up & Concentration
G-Biosciences offers several unique products for DNA clean up, PCR clean up and the clean up and concentration of nucleic acids. The kits use an array of techniques, including dialysis, resin binding and gel filtration to remove unwanted reagents from the nucleic acid samples. For concentration of DNA samples, the use of resin binding columns and gel electrophoresis with subsequent gel extraction can concentrate nucleic acid samples.
DNA Clean Up
Genomic Tube-O-DIALYZER™ rapidly dialyzes >100kb genomic DNA samples to remove small DNA, salts, RNA and other contaminants with minimal hands-on manipulation preserving the quality of large DNA templates.
GET™ CLEAN DNA kit uses our high binding affinity GET™ Spin Columns to remove salts, enzymes, unincorporated nucleotides, radiolabels, and primer-dimers from any DNA preparation of 100bp to >20kb. GET™ Spin Columns has an enhanced binding affinity for DNA, thus eliminating loss or damage of DNA.
Our range of SpinOUT™ columns feature:
- SpinOUT™ For PCR for PCR Clean Up to remove salts, radioisotopes, dyes, primers and deoxynucleotides (dNTPs) from PCR products
- SpinOUT™ GT-600 and SpinOUT™ GT-1200, are ideal for removing excess unincorporated dye terminators, freeing nucleotides from sequencing and labeling reactions. The are a range of sized gel filtration columns for the purification and clean up of DNA fragments >10 bases (GT-600) and 20 bases (GT-1200)
- The SpinOUT™ G-Acryl gel filtration columns are acrylamide based resins and are suitable for purification and clean up of DNA fragments >10 bases (G-Acryl 600) and 20 bases (G-Acryl-1200)
DNA Gel Extraction
G-CAPSULE™ is a simple electroelution device that excises DNA or nucleic acid bands and elutes your sample in a final volume of ~30µl. No requirement for heating or additional chemicals.
GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.