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Antibody Fragmentation

Download the Antibody Production Handbook for Complete Product Listing

 

For the generation of Fab and F(ab)2 fragments for IgG antibodies

The F(ab)2 Fragmentation kits are designed for the generation and isolation of F(ab)2 fragments from IgG molecules.   The kit utilizes our Immobilized Pepsin resin.  Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (Ig..
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The Fab & F(ab)2 Fragmentation of Mouse IgG1 kit is designed for the generation and isolation of Fab and F(ab)2 fragments from mouse IgG1 molecules.  The kit utilizes our Immobilized Ficin resin.  Ficin (or Ficain) (~25,000Da) is a cysteine protease enzyme (EC 3.4.22.3) isolated fro..
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The Fab Fragmentation kits are designed for the generation and isolation of Fab fragments from IgG molecules.    The kits utilize our Immobilized Papain resin.  Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G mole..
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Model:
Ficin (or Ficain) is a cysteine protease enzyme (EC 3.4.22.3) isolated from fig latex is that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. Ficin is typically used to cleave mouse IgG1 as this are difficult to cleave with papain and pepsin. In the..
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Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region.  This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain.  The papain-digested antibody is unable to p..
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Pepsin is a proteolytic enzyme that is routinely used for the generation of F(ab)2 fragments from immunoglobulin G (IgG).  The pepsin has the ability to cleave the heavy chains near the hinge region. One or more of the disulfide bonds that join the heavy chains in the hinge region are preserved..
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