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PCR Reagents

Download the Molecular Biology Handbook for Complete Product Listing

 

G-Biosciences offers a selection of affordable polymerases and dNTPS for performing PCR. These are high quality enzymes and dNTPs for successful and reproducible PCR. The following enzymes are offered and all enzymes are avaialbe as a convenient 2X Mastermix containing the enzyme, dNTPS, salts and reaction buffers. Just add DNA template and primers.

  • Taq DNA Polymerase that is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. Supplied with a 10X PCR Buffer and also available as a Taq DNA Polymerase 2X Mastermix.
  • PFU DNA polymerase has superior thermostability and proofreading properties compared to other thermostable polymerase. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min at 70~75°C. Also possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. A 2X MasterMix Pfu DNA Polymerase is available
  • Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. A 2X MasterMix Taq Plus DNA Polymerase is available.

The deoxynucleotides (dNTPs) are supplied as an equimolar mixture of dATP, dCTP, dGTP and dTTP and are available at 10 and 25mM concentrations or as a set of individual dNTPS or individually at 100mM.

In addition to the polymerases and dNTPS, several kits are offered for the extraction of DNA templates from a large selection of tissues and specimens and several products for PCR clean up following the PCR. 

Pfu DNA Polymerase Mastermix [2X] is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes..
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Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75&d..
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The SpinOUT™ PCR columns are spin format desalting columns that have the ability to remove salts, radioisotpes, dyes, primers and  deoxynucleotides (dNTPs) for nucleic acids following PCR.  Two sizes are available:   SpinOUT™ PCR-20: For rapid purification of PCR ..
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Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yield..
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Taq Plus Mix [2X] is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to highly ..
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The dNTP Mixes are premixed, ready-to-use aqueous solution at pH7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 or 25mM.  The solutions are greater than 99% purity, free from both human and E.coli DNA and are DNase and RNase free. The use of a dNTP mix, as opposed ..
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The dNTP set consists of aqueous 100mM solutions at pH7.0 of dATP, dCTP, dGTP or dTTP. The dNTP set offers maximum flexibility in the preparation of reaction mixes for different applications. dATP, dCTP, dGTP or dTTP are also sold separately. Supplied as 250µl (25µmol) aliquots. Ap..
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Gene/Protein:
2X Taq Mix is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. This pre-mixed formulation saves time and r..
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Gene/Protein:
Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus.  The molecular weight of the recombinant protein is 94kD..  The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°..
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