PCR Reagents
G-Biosciences offers a wide selection of affordable regular and high-fidelity DNA polymerases and deoxynucleotides (dNTPs) for performing PCR. These are high quality enzymes and dNTPs for successful and reproducible PCR.
I) DNA Polymerases:
- Taq DNA Polymerase possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity that can amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C.
- PFU DNA polymerase has superior thermostability and proofreading properties compared to Taq DNA polymerase. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min at 70~75°C. Also possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors.
- Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity.
- Hot start Taq DNA Polymerase is chemically modified that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity.
- High fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This unique Polymerase amplifies DNA with high processivity in highly TA- or GC-rich regions.
- Hot start high-fidelity DNA Polymerase is chemically modified that possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities.
II) DNA Polymerases 2X Master Mix:
- Taq DNA Polymerase 2X master mix is ready to use premix which contains Taq DNA Polymerase, dNTPs and optimized buffer with stabilizers. Taq DNA Polymerase possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity.
- Pfu DNA Polymerase 2X master mix is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination.
- Taq Plus DNA Polymeras 2X master mix is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added.
- Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs and optimized buffer with stabilizers.
- High-fidelity DNA Polymerase 2x master mix is ready to use premix which contains high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers.
- Hot start high-fidelity DNA Polymerase 2x master mix is ready to use premix which contains hot start high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers.
III) Deoxynucleotides (dNTPs):
The dNTPs are supplied as
- an equimolar mixture of dATP, dCTP, dGTP and dTTP that are available at 10 and 25mM concentrations
- a set of individual dNTPS
- individually at 100mM.
Related Products:
In addition to the DNA polymerases and dNTPS, several kits are offered for the
- Extraction of Genomic DNA from a large selection of tissues and specimens
- Plasmid Purification & Colony Screening
- DNA Size Selection & PCR Clean up with Magnetic Beads
- PCR clean up following the PCR.
G-biosciences hot start Taq DNA Polymerase is chemically modified that possesses a 5´→3´ polymerase activity and a 5´-flap endonuclease activity. Hot start Taq DNA Polymerase reduces non-specific amplification during PCR setup and increases PCR specificity and sensitivity.
Pr..
$0.00
![Pfu DNA Polymerase MasterMix [2X]](https://cdn.gbiosciences.com/cache/786-817-500x500.png "Pfu DNA Polymerase MasterMix [2X]")
Gene/Protein:
Pfu DNA Polymerase Mastermix [2X] is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTPs, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes..
$0.00
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kD. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min (70~75&d..
$0.00
The SpinOUT™ PCR columns are spin format desalting columns that have the ability to remove salts, radioisotpes, dyes, primers and deoxynucleotides (dNTPs) for nucleic acids following PCR. Two sizes are available:
SpinOUT™ PCR-20: For rapid purification of PCR ..
$0.00
Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yield..
$0.00
![Taq PLUS DNA Polymerase MasterMix [2X]](https://cdn.gbiosciences.com/cache/786-1964-500x500.jpg "Taq PLUS DNA Polymerase MasterMix [2X]")
Taq Plus Mix [2X] is a premixed, ready-to-use solution containing Taq Plus DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Plus Mix contributes to highly ..
$0.00
Gene/Protein:
G-Biosciences DNA Size Selection & PCR Cleanup Magnetic beads are suitable for PCR clean-up and DNA fragment size selection from 150 bp to 1000 bp and even larger. They remove excess nucleotides, salts, enzymes, and other impurities with a simple washing process to obtain purified fragments, whi..
$0.00
Gene/Protein:
DNA Size Selection Magnetic beads are suitable for DNA fragment size selection from 150 bp to 1000 bp and even larger. They remove excess nucleotides, salts, enzymes, and other impurities with a simple washing process to obtain purified fragments, which can be directly used in downstream applic..
$0.00
![dNTP Mix [10 & 25mM]](https://cdn.gbiosciences.com/cache/dNTPs-500x500.jpg "dNTP Mix [10 & 25mM]")
The dNTP Mixes are premixed, ready-to-use aqueous solution at pH7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 or 25mM. The solutions are greater than 99% purity, free from both human and E.coli DNA and are DNase and RNase free. The use of a dNTP mix, as opposed ..
$0.00
Gene/Protein:
The dNTP set consists of aqueous 100mM solutions at pH7.0 of dATP, dCTP, dGTP or dTTP. The dNTP set offers maximum flexibility in the preparation of reaction mixes for different applications. dATP, dCTP, dGTP or dTTP are also sold separately. Supplied as 250µl (25µmol) aliquots.
..
$0.00
G-biosciences high-fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This Polymerase has the fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significan..
$0.00
Gene/Protein:
G-biosciences high-fidelity DNA Polymerase 2x master mix is ready to use premix which contains high fidelity DNA Polymerase, dNTPs and optimized buffer with stabilizers. The high-fidelity DNA Polymerase possesses 5´→3´ polymerase and 3´→5´ exonuclease (proofreading)..
$0.00