G-Biosciences offers a selection of affordable polymerases and dNTPS for performing PCR. These are high quality enzymes and dNTPs for successful and reproducible PCR. The following enzymes are offered and all enzymes are avaialbe as a convenient 2X Mastermix containing the enzyme, dNTPS, salts and reaction buffers. Just add DNA template and primers.
- Taq DNA Polymerase that is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. Supplied with a 10X PCR Buffer and also available as a Taq DNA Polymerase 2X Mastermix.
- PFU DNA polymerase has superior thermostability and proofreading properties compared to other thermostable polymerase. It can amplify DNA target up to 2kb. The elongation velocity is 0.2~0.4kb/min at 70~75°C. Also possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. A 2X MasterMix Pfu DNA Polymerase is available
- Taq Plus DNA Polymerase is a mixture of Taq DNA Polymerase and Pfu, a proofreading DNA Polymerase, which allows for the amplification of long templates, up to 20kb, with high fidelity. A 2X MasterMix Taq Plus DNA Polymerase is available.
The deoxynucleotides (dNTPs) are supplied as an equimolar mixture of dATP, dCTP, dGTP and dTTP and are available at 10 and 25mM concentrations or as a set of individual dNTPS or individually at 100mM.
In addition to the polymerases and dNTPS, several kits are offered for the extraction of DNA templates from a large selection of tissues and specimens and several products for PCR clean up following the PCR.