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Amino Acid Side Chain Modifiers

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

A range of chemicals that modify amino acid side chains to block side chain interactions, change the charge or modify them to be more favorable for other reactions.

4-Vinylpyridine is used as a derivatizing reagent for free thiols such as GSH. It is used in the Glutathione colorimetric assay to remove reduced GSH, so that the oxidized Glutathione (GSSG) concentration can be measured. 4-Vinylpyridine is a better derivatizing reagent for GSH when compared to N-et..
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L-Cysteine hydrochloride salt is routinely used with Ellman's Reagent assays as a sulfhydryl standard. In addition, it is also used as a supplement for protein refolding experiments.   Features CAS #: 7048-04-6 Formula: HSCH2CH(NH2)COOH•HCl•H2O Molecular weight: 175.63 Pu..
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HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.   Features of HPG: • Arginine-specific—reacts specifically with arginine residues under mild conditions (p..
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TNBS (2,4,6-trinitrobenzene sulfonic acid) is a highly sensitive and rapid chemical used to quantitate the free amino groups.  The reaction of TNBS with primary amines generates a highly chromogenic product that can be readily measured at 335nm. Supplied as a 1% solution in methanol.   ..
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1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger’s reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary a..
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G-Biosciences Carboxylation Kit With Citraconic Anhydride is designed for reversible blocking of primary amines so that directed conjugation or labeling can be performed. Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate. The link..
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Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate.   The linkage is stable at neutral to alkaline pH (pH >7) and at acidic conditions (pH 4) the amide linkage is rapidly hydrolyzed to release the citraconic acid and free..
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Oxidative Modification Kit is designed for the modification of glycoproteins to reactive aldehydes for amine- and hydrazide- labeling. Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye g..
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G-Biosciences Protein Acetylation Kit is designed for modifying proteins by blocking their primary amines. The NHS ester group of Sulfo NHS Acetate reacts with primary amines of protein in non-amine containing buffers at pH 7-9. After reaction, the amine is irreversibly capped with an acyl group. M..
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Reactive Amination Kit is designed to prepare amine-reactive esters of carboxylate groups for chemical labeling. Sulfo-NHS is used to generate amine reactive esters from carboxylate groups and is primarily used to increase the efficiency of the carbodiimide EDC cross‐linking.   Features: p..
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SATA is used to add protected sulfhydryls to primary amines. Kit related to SATA is also available [Sulfhydration Kit With SATA] Features Chemical Name: N-Succinimidyl S-acetylthioacetate CAS Number: 76931-93-6 Molecular Weight: 245.25 Reacts primarily with primary amines ..
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Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1).  The reactive aldehyde groups are used in chemical conjugation procedures or detection of carbohydrates.  F..
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