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Amino Acid Side Chain Modifiers

Download the Crosslinkers Handbook & Selection Guide            Download the Protein Labeling & Conjugation Handbook

 

A range of chemicals that modify amino acid side chains to block side chain interactions, change the charge or modify them to be more favorable for other reactions.

4-Vinylpyridine is used as a derivatizing reagent for free thiols such as GSH. It is used in the Glutathione colorimetric assay to remove reduced GSH, so that the oxidized Glutathione (GSSG) concentration can be measured. 4-Vinylpyridine is a better derivatizing reagent for GSH when compared to N-et..
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Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate.   The linkage is stable at neutral to alkaline pH (pH >7) and at acidic conditions (pH 4) the amide linkage is rapidly hydrolyzed to release the citraconic acid and ..
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L-Cysteine hydrochloride salt is routinely used with Ellman's Reagent assays as a sulfhydryl standard. In addition, it is also used as a supplement for protein refolding experiments.   Features CAS #: 7048-04-6 Formula: HSCH2CH(NH2)COOH•HCl•H2O Molecular weight: ..
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HPG (p-hydroxyphenylglyoxal) reacts to specifically modify arginine residues under mild conditions to yield spectrophotometrically measurable signal for amino acid detection.   Features of HPG: • Arginine-specific—reacts specifically with arginine residues under mild conditi..
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  Features Chemical Name: N-Succinimidyl S-acetylthioacetate CAS Number: 76931-93-6 Molecular Weight: 245.25 Reacts primarily with primary amines Adds protected sulfhydryl residues Sulfhydryl group can be used in coupling reactions S..
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Sodium metaperiodate, or sodium m-periodate, is a mild oxidant that is routinely used for the conversion of cis-glycol groups in carbohydrates to reactive aldehdye groups (Figure 1).  The reactive aldehyde groups are used in chemical conjugation procedures or detection of carbohydrates.  F..
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Features Chemical Name: N-Hydroxysulfosuccinimide Reacts primarily with primary amines Water soluble Molecular weight: 217.13   Applications Ideal for cross-linking, chemical labeling and solid support immobilization Increase efficiency of E..
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Features Chemical Name: Sulfosuccinimidyl acetate Reacts primarily with primary amines Reacts at pH 7-9 Water soluble Molecular weight: 259.17   Applications Blocks primary amines by acylation    ..
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TNBS (2,4,6-trinitrobenzene sulfonic acid) is a highly sensitive and rapid chemical used to quantitate the free amino groups.  The reaction of TNBS with primary amines generates a highly chromogenic product that can be readily measured at 335nm. Supplied as a 1% solution in methanol.  ..
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Features Chemical Name: 2-Iminothiolane hydrochloride Molecular weight: 137.63 Mild conditions: pH 7-10, 25°C Soluble in water   Applications For the addition of sulfhydryls to primary amines For the preparation of disulfide bridges or g..
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