Protein Quantitation Assays

Protein assays, most notably quantitation or estimation assays, for determining protein concentration are one of the most widely used methods in life science research. Protein estimation of protein concentration is necessary in protein purification, electrophoresis, cell biology, molecular biology, and other research applications. We offer a unique selection of protein estimation assays that are improvements on the Biuret, Lowry, BCA and Bradford assays.  Each assay has its own benefits, however the NI™ (Non-Interfering™) Protein Assay and CB X™ Protein Assay overcome all interfering agents.

Although there are a wide variety of protein assays available, none of the assays can be used without first considering their suitability for the application. Each method has its own advantages and limitations and often it is necessary to obtain more than one type of protein assay for research applications.For additional information on protein assays, read the introduction to  "Protein Assays Handbook".

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Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions, however is dramatically affected by the presence of..

CB™ Protein Assay

An improved Coomassie Dye based protein assay based on the Bradford Protein Assay (1). This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a sin..

Micro Bicinchoninic Acid (BCA) Protein Assay

The Micro Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the Bicinchoninic Acid (..

Protein dotMETRIC™ Assay

Rapidly, mix 1µl protein sample with the supplied Dilution Buffer and apply 1µl of the solution to the test strip by point-of-contact capillary action. Under the assay's specific buffer co..

Protn-Latex™

Protn-Latex™ is a simple and reliable method for estimation of protein contamination in latex. Protein estimation can be performed either on the production site or in any laboratory with a minim..

SPN™ Protein Assays

A novel protein assay that is suitable for single sample or high throughput protein estimation. The SPN™ and SPN™-htp protein assays are rapid assays that are suitable for as little as 0.5..

Bicinchoninic Acid (BCA) Protein Assay

The Bicinchoninic Acid (BCA) Protein Assay, also known as the Smith Assay, is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions.   The Bi..

CB X™ Protein Assay

Protein assays are routinely used in many research fields to estimate proteins in a vast array of buffers and conditions. A major problem for researchers is to select a protein assay from the vast sel..

NI™ (Non-Interfering™) Protein Assay

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  A highly sensitive, colorimetric protein assay that o..