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Oxidative Stress

Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect.

G-Biosciences Antioxidant Capacity Assays are useful for the determination of this parameter in different sample types such as food and beverages or plasma.

There are two types of mechanisms to determine the antioxidant capacity on different matrices: electron-transfer (E-T) methods, which measure the ability of an antioxidant to reduce an oxidant, with a change of color (ABTS, FRAP, DMPD) and hydrogen atom mediated (HAT) methods, which measure the ability of an antioxidant to eliminate free radicals through the donation of a hydrogen atom (they normally quantify peroxyl radicals; ORAC).

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe2+) ions to ferric (Fe3+) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the ..
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