Fractionation and Enrichment Kits

The analysis of a proteome is often inhibited by the vast amount of proteins, with large abundant proteins inhibiting the signal of lower abundance and often more interesting proteins.  Researchers overcome this problem by using fractionation, however inconsistencies in techniques and buffers often result in a lack of reproducibility.
G-Biosciences offers a wide selection of fractionation kits for processing samples from cells, tissues, bacteria, yeast, plants, and other types of samples. A selection of sample preparation accessories and supplies are also available. The following kits, accessories, and supplies are suitable for the analysis of proteins using electrophoresis and other biochemical techniques.

The FOCUS line of products allow for the fractionation of a large selection of biological samples into a multitude of different fractions and these resulting fractions are compatible with 2D electrophoresis and subsequent protein identification techniques.

Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT has been specifically developed for substantial removal of albumin from such samples.

We also offer a large variety of accessories to aid in protein fractionation and enrichment procedures.

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Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophore..

Concanavalin A (Con A) Agarose

Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method.  Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean)...


A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extrac..

FOCUS™ Cytoplasmic & Nuclear Proteins

The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible..

FOCUS™ Glycoprotein

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.   FOCUS™ Glycoprotein kit isolates gly..

FOCUS™ Membrane Proteins

FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane..

FOCUS™ PhosphoRich™

FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotei..

FOCUS™ Signal Proteins

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycol..

FOCUS™ Soluble & Insoluble

A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The ..


2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins, however the greatest challenge in protein identification is achieving suitable resolution of proteins. ..

Guanidine Hydrochloride

A selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The..

HOOK™ Cell Surface Protein Isolation

HOOK™ Cell Surface Protein Isolation kit uses G-Biosciences HOOK™ biotin labeling and purification technology in conjunction with our Mammalian Cell PE LB™ lysis buffer to convenient..