Fractionation and Enrichment Kits

The analysis of a proteome is often inhibited by the vast amount of proteins, with large abundant proteins inhibiting the signal of lower abundance and often more interesting proteins.  Researchers overcome this problem by using fractionation, however inconsistencies in techniques and buffers often result in a lack of reproducibility.
G-Biosciences offers a wide selection of fractionation kits for processing samples from cells, tissues, bacteria, yeast, plants, and other types of samples. A selection of sample preparation accessories and supplies are also available. The following kits, accessories, and supplies are suitable for the analysis of proteins using electrophoresis and other biochemical techniques.

The FOCUS line of products allow for the fractionation of a large selection of biological samples into a multitude of different fractions and these resulting fractions are compatible with 2D electrophoresis and subsequent protein identification techniques.

Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT has been specifically developed for substantial removal of albumin from such samples.

We also offer a large variety of accessories to aid in protein fractionation and enrichment procedures.
 

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PhosphoQuant™

PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrol..

AlbuminOUT™

Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophore..

Concanavalin A (Con A) Agarose

Concanavalin A (Con A) Agarose consists of Con A coupled to 4% agarose by the cyanogen bromide method.  Con A is a tetrameric metalloprotein lectin isolated from Canavalia ensiformis (jack bean)...

DTT

High quality proteomic grade DTT, in various sizes, is available for your convenience.     Dithiothreitol (DTT) DTT is a common reducing agent used for the cleavage of disulfide ..

EZ-Grind™

A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extrac..

FOCUS™ Cytoplasmic and Nuclear Proteins

The FOCUS™ Cytoplasmic and Nuclear Protein Fractionation Kit is supplied with a strong chaotropic extraction buffer to solubilize both cytoplasmic and nuclear proteins, which is fully compatible..

FOCUS™ Global Fractionation

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into e..

FOCUS™ Glycoprotein

FOCUS™ Glycoprotein kit is based on lectin binding of specific glycoproteins with terminal α-D mannosyl and α-D glycosyl proteins.   FOCUS™ Glycoprotein kit isolates..

FOCUS™ Membrane Proteins

FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane..

FOCUS™ Mitochondria

Protocols.io provides an interactive version of this protocol where you can discover and share optimizations with the research community.  Specifically designed for the isolation of intact mit..

FOCUS™ PhosphoRich™

FOCUS™ PhosphoRich™ is a ready-to-use kit that enriches phosphorylated proteins and phosphopeptides from complex biological samples. The kit contains spin columns that have a phosphoprotei..

FOCUS™ Signal Proteins

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycol..