The analysis of a proteome is often inhibited by the vast amount of proteins, with large abundant proteins inhibiting the signal of lower abundance and often more interesting proteins. Researchers overcome this problem by using fractionation, however inconsistencies in techniques and buffers often result in a lack of reproducibility.
G-Biosciences offers a wide selection of fractionation kits for processing samples from cells, tissues, bacteria, yeast, plants, and other types of samples. A selection of sample preparation accessories and supplies are also available. The following kits, accessories, and supplies are suitable for the analysis of proteins using electrophoresis and other biochemical techniques.
The FOCUS™ line of products allow for the fractionation of a large selection of biological samples into a multitude of different fractions and these resulting fractions are compatible with 2D electrophoresis and subsequent protein identification techniques.
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophoresis and other studies. AlbuminOUT™ has been specifically developed for substantial removal of albumin from such samples.
We also offer a large variety of accessories to aid in protein fractionation and enrichment procedures.
PhosphoQuant™ is specifically designed for quick and reliable determination of whether a purified protein is phosphorylated and the extent of phosphorylation. The assay is based on the alkaline hydrol..
Samples that contain a large abundance of albumin, such as plasma and cerebrospinal fluid, tend to mask identification and discovery of other less abundant proteins in two dimensional gel electrophore..
A highly efficient grinding resin that is pre-aliquoted into 1.5ml grinding tubes and is supplied with matching pestles. The resin is designed for optimal grinding of biological samples for the extrac..
Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycol..
A complete kit for the selective preparation of soluble (hydrophilic) and insoluble (hydrophobic) proteins from mammalian tissues and cells, plants, yeast, bacteria, and other biological samples. The ..