RAPIDstain™
An ultra sensitive and ready-to-use protein stain based on Coomassie dye. No mixing or preparation is involved. No fixation step is needed. Simply rinse gel, add RAPID
stain™ and protein bands develop within 5-10 minutes, reaching a maximum visibility in an hour. Mini-gels can be stained in 10 minutes.
RAPID
stain™ only stains protein, leaving a crystal-clear background resulting in high band visibility. The gels require no destaining. The protein band intensity is enhanced by simply rinsing with water.
RAPID
stain™ has the sensitivity to detect as little as 4-8ng BSA. Staining shows a linear response for densitometric gel analysis and produces sharp scanning or photographic results.
RAPID
stain™ is supplied in 1 liter, sufficient reagent to stain up to 50 mini gels (8 x 10cm) or as a 1 gallon size for staining up to 200 mini gels
FEATURES
- Sensitive, 4-8ng BSA.
- Saves time, band develops within 5-10 minutes.
- Uses a single step method.
- No methanol or acetic acid destaining agents.
- Works with native PAGE, SDS-PAGE, isoelectric focusing, or 2D gels.
APPLICATIONS
- Staining of protein electrophoresis gels.
- Suitable for native, denaturing, isoelectric focusing, and 2D gels.
References
- Caswell, C.C. et al (2007) Mol Microbiol. 64:1319.
- Han, R. et al (2006) Mol Microbiol. 61:351.
- Han, R. et al (2006) Appl Microbiol Biotechnol. 72:109.