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Agarose II (Low Melting)

Agarose II (Low Melting)
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Agarose II (Low Melting)
Agarose II (Low Melting)
Catalog Description Size Price (USD) Qty
RC-007Agarose II (Low Melting agarose)25g$201.00
RC-008Agarose II (Low Melting agarose)100g$469.00
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  • Description
    Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice. 
     
    Specifications
    • Moisture: ≤ 7%
    • Ash: ≤ 0.4%
    • EEO (electroendosmosis): ≤ 0.12
    • Sulfate: ≤ 0.12%
    • Clarity 1.5% (NTU): ≤ 4
    • Gel Strength 1.5% (g/cm2): ≥ 500
    • Gelling Temperature 1.5% (ºC): 24-28
    • Melting Temperature 1.5% (ºC): ≤ 65.5
    • DNase/ RNase Activity: None Detected
    • DNA Resolution ≥1000bp: Finely resolved
    • Gel background: Very low
     
    Appropriate Agarose Concentrations
    GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER
    0.75 500-20,000bp 500-12,000bp
    1.00 300-16,000bp 300-8,000bp
    1.25 250-10,000bp 200-4,000bp
    1.50 200-5,000bp 150-3,000bp
    1.75 100-2,500bp 100-2,000bp
    2.0 50-1,500bp 50-1,000bp

     


    Features

    • Lower gel strength compared to standard agarose
    • Higher clarity
    • Easy to handle

     


    Applications

    • Electrophoresis of DNA Fragments >1000bp
    • Tissue and Cell Culture
    • Viral Plaque Assays

     

     

  • Reviews
Agarose II has a low melting and gelling temperature compared to standard agaroses.  The low melting temperature allows for the recovery of undamaged nucleic acids below the denaturation temperature.  The low gelling temperature ensures that the agarose will be in a liquid state at a temperature range where in-gel manipulations can be performed without prior extraction from the agarose slice. 
 
Specifications
  • Moisture: ≤ 7%
  • Ash: ≤ 0.4%
  • EEO (electroendosmosis): ≤ 0.12
  • Sulfate: ≤ 0.12%
  • Clarity 1.5% (NTU): ≤ 4
  • Gel Strength 1.5% (g/cm2): ≥ 500
  • Gelling Temperature 1.5% (ºC): 24-28
  • Melting Temperature 1.5% (ºC): ≤ 65.5
  • DNase/ RNase Activity: None Detected
  • DNA Resolution ≥1000bp: Finely resolved
  • Gel background: Very low
 
Appropriate Agarose Concentrations
GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER
0.75 500-20,000bp 500-12,000bp
1.00 300-16,000bp 300-8,000bp
1.25 250-10,000bp 200-4,000bp
1.50 200-5,000bp 150-3,000bp
1.75 100-2,500bp 100-2,000bp
2.0 50-1,500bp 50-1,000bp

 


Features

  • Lower gel strength compared to standard agarose
  • Higher clarity
  • Easy to handle

 


Applications

  • Electrophoresis of DNA Fragments >1000bp
  • Tissue and Cell Culture
  • Viral Plaque Assays

 

 

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