Agarose IV has higher gel strength compared to standard agaroses and is therefore suitable for the resolution of small DNA fragments and PCR products.
For improved resolution we recommend storing gel at 4°C for 30 minutes before use.
Specifications
|
Moisture
|
≤ 7%
|
|
Ash
|
≤ 0.35%
|
|
EEO ((Electroendosmosis)
|
≤ 0.12
|
|
Sulfate
|
≤ 0.11%
|
|
Clarity 1.5% (NTU)
|
≤ 5
|
|
Gel Strength 1.5% (g/cm2)
|
≥ 600
|
|
Gel Strength 3% (g/cm2)
|
≥ 1500
|
|
Gelling Temperature 3% (ºC)
|
≤ 35.5
|
|
Melting Temperature 3% (ºC)
|
≤ 80
|
|
DNase/ RNase Activity
|
None Detected
|
|
Gel background
|
Very low
|
Appropriate Agarose Concentrations
| GEL CONCENTRATION (%) |
1X TAE BUFFER |
1X TBE BUFFER |
| 1.8 |
400-1,200bp |
100-1,200bp |
| 3.0 |
150-800bp |
40-700bp |
| 4.0 |
20-500bp |
20-200bp |
| 5.0 |
10-300bp |
<100bp |
FEATURES
-
High resolution of short DNA fragments
-
High resolution of small PCR products
-
Improved clarity for greater visualization
-
Improved handling compared to competitor's. Less gel breaking and cracking
-
High gel strength ideal for blotting
APPLICATIONS