A 1µl Assay For Rapid Estimation of DNA, RNA and Oligo Concentration
NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an alternative to spectrophotometry as no expensive equipment or cuvettes are necessary. NUCLEIC dotMETRIC™ uses as little as a microliter of your sample and provides permanent results in minutes.
Samples in the range of 10µg/µl to 1ng/µl can be measured accurately regardless of the isolation method or storage buffer. Oligonucleotides as short as 16 bases have successfully been measured with NUCLEIC dotMETRIC™. Measurements are not affected by protein contamination in the sample. The lower detection limit of the system is 1-2ng/µl making NUCLEIC dotMETRIC™ one of the most sensitive methods available for measuring nucleic acids.
Two separate protocols are offered for measuring nucleic acid concentration; either by measuring the diameter of nucleic acid spots or comparing the color density of the nucleic acid spots with that of a set of known standards.
SIMPLE TO USE
Samples are diluted with dilution buffer and 1-5µl spotted onto the NUCLEIC Test Strip, which are developed with NUCLEIC Dye in under 2 minutes. The dotMETRIC™ scale and spot diameter are compared for accurate nucleic acid concentrations. Alternatively, the color density of the nucleic acid spots is compared to the NUCLEIC standard strip. No spectrophotometers or cuvettes required.
EASY-TO-USE SPOT APPLICATION DEVICE
For increased reproducibility and test reliability the kits are supplied with an optional Spot Application Device. This allows application of samples using fixed volume capillary tips, simplifying the application of the nucleic acid solution and improving the reliability of results.
RESISTANT TO MOST COMMON LABORATORY AGENTS
The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts and other common laboratory agents.
Spot Application Device (Cat. # 786-63): Simplifies the application of samples and improves reliability.
Application Glass Capillary Tips (Cat. # 786-23): 1µl Application glass capillary tips for use with Spot Application Device.
Sample Application (pipette) Tips (Cat. # 786-64): 1-10µl pipettor tips for standard pipettes.
Developing Trays (Cat. # 786-24): Tray for developing NUCLEIC dotMETRIC™ test strips.