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NUCLEIC dotMETRIC™ Assay

  • Protocol, Manual or Handbook Protocol MSDS, Material safety data sheet MSDS CofA, Certificate of Analysis Certificate of Analysis
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  • 786-23
  • 1µl Application Glass Capillary Tips

  • 100
  • $21.00
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  • Ambient
  • MSDS, Material safety data sheet
  • 786-24
  • Developing Trays

  • 2
  • $17.00
  •   Add to Cart
  • Ambient
  • MSDS, Material safety data sheet
  • 786-63
  • dotMETRIC™ Spot Application Device

  • 1
  • $140.00
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  • Ambient
  • MSDS, Material safety data sheet
  • 786-61
  • NUCLEIC dotMETRIC™ Basic Kit, with Spot Application Device

  • >300 assays
  • $364.00
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  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet
  • 786-60
  • NUCLEIC dotMETRIC™ Basic Kit

  • >300 assays
  • $237.00
  •   Add to Cart
  • Ambient
  • Protocol, Manual or Handbook MSDS, Material safety data sheet
  • 786-64
  • Sample Application (pipette) Tips

  • 96 tips
  • $20.00
  •   Add to Cart
  • Ambient
  • MSDS, Material safety data sheet

A 1µl Assay For Rapid Estimation of DNA, RNA and Oligo Concentration
 
NUCLEIC dotMETRIC™ kit provides a quick and accurate method for detecting and measuring DNA, RNA and oligonucleotides. The system uses a unique combination of proprietary reagents and test strips and is an alternative to spectrophotometry as no expensive equipment or cuvettes are necessary. NUCLEIC dotMETRIC™ uses as little as a microliter of your sample and provides permanent results in minutes.
 
Samples in the range of 10µg/µl to 1ng/µl can be measured accurately regardless of the isolation method or storage buffer. Oligonucleotides as short as 16 bases have successfully been measured with NUCLEIC dotMETRIC™. Measurements are not affected by protein contamination in the sample. The lower detection limit of the system is 1-2ng/µl making NUCLEIC dotMETRIC™ one of the most sensitive methods available for measuring nucleic acids.
 
Two separate protocols are offered for measuring nucleic acid concentration; either by measuring the diameter of nucleic acid spots or comparing the color density of the nucleic acid spots with that of a set of known standards.
 
SIMPLE TO USE
Samples are diluted with dilution buffer and 1-5µl spotted onto the NUCLEIC Test Strip, which are developed with NUCLEIC Dye in under 2 minutes. The dotMETRIC™ scale and spot diameter are compared for accurate nucleic acid concentrations. Alternatively, the color density of the nucleic acid spots is compared to the NUCLEIC standard strip. No spectrophotometers or cuvettes required.
 
EASY-TO-USE SPOT APPLICATION DEVICE
For increased reproducibility and test reliability the kits are supplied with an optional Spot Application Device. This allows application of samples using fixed volume capillary tips, simplifying the application of the nucleic acid solution and improving the reliability of results.
 
RESISTANT TO MOST COMMON LABORATORY AGENTS
The assay is unaffected by the presence of common laboratory agents, such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts and other common laboratory agents.
 
ACCESSORIES
Spot Application Device (Cat. # 786-63): Simplifies the application of samples and improves reliability.
 
Application Glass Capillary Tips (Cat. # 786-23): 1µl Application glass capillary tips for use with Spot Application Device.
 
Sample Application (pipette) Tips (Cat. # 786-64): 1-10µl pipettor tips for standard  pipettes.
 
Developing Trays (Cat. # 786-24): Tray for developing NUCLEIC dotMETRIC™ test strips.
FEATURES
    • Sensitive: Detect 1-5ng/μl nucleic acid within 3 minutes.
    • Sample Economy: Use as little as 1µl of sample.
    • Rapid Assay: Takes 2-3 minutes and can assay as little as 1-5ng.
    • Suitable for 300 assays.
    • Supplied with optional Spot Application Device.
APPLICATIONS
    • For rapid estimation of nucleic acid concentration, including DNA, RNA & oligonucleotides.
    • For when a limited amount of sample is available for analysis, requires only 1µl sample.
References

ISO 9001:2008 Certified


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