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G-Biosciences - Reversible Zinc Stain™
5 Minute Reversible Zinc Stain
 
The Reversible Zinc StainTM is a single step stain for the rapid detection of proteins resolved by PAGE (native gels or SDS denatured gels). No de-staining is necessary.
 
The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white, while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image of the gel is produced; clear protein bands are detected against a semi-opaque white polyacrylamide background. Protein bands are visualized in as little as 10-15 minutes.
 
The sensitivity of the Reversible Zinc StainTM is 8-12ng and does not interfere with electroelution of proteins or alter their biological properties. Gels stained with the Reversible Zinc StainTM can be de-stained in 5 minutes before the transfer or electroelution of proteins.
 
This stain works with native as well as SDS denatured gels and gels containing Glycine, Tricine and a variety of primary-amine containing buffers.
 
Tissue extract loaded on 4%-20% gradient gel and
developed with Reversible Zinc StainTM. Gel was
Protein load, 0.25mg to 5mg per well. Gel was
photographed against a blue background.
 
FEATURES
  • Reversible, simple, fast, and sensitive.
  • High contrast protein bands.
  • Works with native and SDS denatured gels and gels containing Glycine and Tricine.
  • Excellent for subsequent protein elution, blotting, sequencing, mass spectroscopy, etc.
APPLICATIONS
  • Suitable for staining of protein gels.
  • Staining suitable for subsequent protein elution, blotting, sequencing, mass spectroscopy, etc.
Catalog #DescriptionSizeShipping
Condition
Price 
786-32ZNReversible Zinc Stain™25 Mini-gels Ambient US $119.00
   
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