5 Minute Reversible Zinc Stain

The Reversible Zinc StainTM is a single step stain for the rapid detection of proteins resolved by PAGE (native gels or SDS denatured gels). No de-staining is necessary.

The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing a zinc metal precipitate in the gel which turns the gel opaque white, while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image of the gel is produced; clear protein bands are detected against a semi-opaque white polyacrylamide background. Protein bands are visualized in as little as 10-15 minutes.

The sensitivity of the Reversible Zinc StainTM is 8-12ng and does not interfere with electroelution of proteins or alter their biological properties. Gels stained with the Reversible Zinc StainTM can be de-stained in 5 minutes before the transfer or electroelution of proteins.

This stain works with native as well as SDS denatured gels and gels containing Glycine, Tricine and a variety of primary-amine containing buffers.

Tissue extract loaded on 4%-20% gradient gel and developed with Reversible Zinc StainTM. Gel was Protein load, 0.25mg to 5mg per well. Gel was photographed against a blue background.

FEATURES

Reversible, simple, fast, and sensitive. High contrast protein bands. Works with native and SDS denatured gels and gels containing Glycine and Tricine. Excellent for subsequent protein elution, blotting, sequencing, mass spectroscopy, etc.

APPLICATIONS

Suitable for staining of protein gels. Staining suitable for subsequent protein elution, blotting, sequencing, mass spectroscopy, etc.