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Phosphatase Assay

Phosphatase Assay
Phosphatase Assay
Phosphatase Assay
Catalog Description Size Price (USD) Qty
786-453Phosphatase Assay kit1,000 Assays$415.00

The Phosphatase Assay kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases.

 

The Phosphatase Assay kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases (Figure).

 

The phosphatases remove the phosphate group to generate p-nitrophenol, which is deprotonated under alkaline conditions to produce p-nitrophenolate that has strong absorption at 405nm.

 

The Phosphatase Assay Kit's components are sufficient for performing up to 1000 assays in 96-well plate format and easily adaptable to cuvettes or 384-well plates.

 


Features

  • A colorimetric, pNPP based assay.
  • Measure phosphatase activity in biological samples.
  • Screen for phosphatase agonists and inhibitors.

 


Applications

  •  For the quantification of phosphatase activity.
  •  To screen for agonists and inhibitors of phosphatases.

 

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