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ML (Modified Lowry) Protein Assay

ML (Modified Lowry) Protein Assay
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ML (Modified Lowry) Protein Assay
ML (Modified Lowry) Protein Assay
ML (Modified Lowry) Protein Assay
ML (Modified Lowry) Protein Assay
ML (Modified Lowry) Protein Assay
Catalog Description Size Price (USD) Qty
786-1075ML Protein Assay with BSA Standard1,000 Assays$120.00
786-1076ML Protein Assay with Non Animal Protein Standard1,000 Assays$120.00
786-1082ML Protein Assay with Pre-Diluted BSA Protein Standard1,000 Assays$148.00
786-1083ML Protein Assay with Bovine ɣ Globulin Standard1,000 Assays$130.00
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  • Description

    G-Biosciences’ ML (Modified Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris (see tables 1 and 2). The assay is supplied with either traditional bovine serum albumin (BSA) protein standard, pre-diluted BSA protein standards, non animal protein standard or bovine ɣ globulin protein standard. 
     

    A CL (Compatible Lowry) Protein Assay is available for samples containing reducing agents (such as dithiothreitol (DTT), ß-mercaptoethanol and TCEP) and a wide variety of commonly used laboratory agents that are known to interfere with Lowry’s protein assays (see tables 1 and 2).
     

    Table-1: A selection of compounds and their maximum concentrations compatible with the ML and CL Protein Assay.

                         Reagent   ML Protein Assay   CL Protein Assay
    Amino Acids Compatible -
    Ammonium Sulfate 0.5M 40%
    β-Mercaptoethanol X 5%, 15%*
    Brij® 35 1% 1%
    Calcium Chloride 0.05M -
    CHAPS 1% 4%
    CHAPSO 1% 1%
    CTAB - 1M
    Deoxycholate 1% -
    Digitonin 0.3% -
    DTT 0.001M 0.1M, 0.35M*
    EDTA 0.025M 0.1M
    Glycerol - 30%
    Guanidine.HCl 0.4M 6M
    Guanidine Thiocynate - 6M
    HEPES - 0.1M
    Hydrochloric Acid 0.5M -
    Imidazole - 0.5M
    Iodoacetamide - 15mM
    Laemmli Buffer (w/5% β-Mercaptoethanol) - Compatible
    NP-40 2% -
    Octaethyleneglycol dodecyl ether 0.2% -
    Octyl Glucoside 1% -
    Phosphate Buffer - 0.2M
    Sarcosyl - 1%
    SDS 10% -
    Sodium Azide 0.05% 0.1M
    Sodium Chloride - 0.5M
    Sodium Hydroxide 0.5M 2.5M
    Sucrose - 30%
    TCEP - 15mM
    Thesit® 1% 2%
    Thiourea - 2M
    Tributylphosphine (TBP) 0.002M -
    Tris (pH 8) 0.1M 0.5M
    Triton® X-100 1% 3%
    Triton® X-114 1% 3%
    Tween® 20 1% 2%
    Urea 4M 8M
    Zwittergent® 3-12 - 1.5M

    -, not tested; X, not compatible; *, two washes (optional).


     

    Table 2: ML & CL Protein Assay are compatible with strong chaotropic extraction buffers

    Buffer Composition
    4M Urea, 1% SDS, 10mM EDTA, 0.8% β-Mercaptoethanol
    6M Urea, 2M Thiourea, 4% CHAPS
    6M Urea, 2M Thiourea, 4% NP-40
    1% Sarcosyl, 0.8 β-Mercaptoethanol, 4M Guanidine Thiocyanate, 10mM EDTA
    6M Urea, 2M Thiourea, 2% CHAPS, 2% ND SB 201
    6M Urea, 2M Thiourea, 2% CHAPS, 2% SB 2 10
     

    Features
    • Protein quantitation in the presence of detergents 
    • Quick 15 minute incubation
    • Increased color stability (color will not change more than 5% in 1 hour or 10% in 2 hours)
    • Linear response 0.2 – 1.5 mg/ml
    • One simple protocol without cumbersome sample preparation or buffer exchange steps
    • Convenient kit provides all necessary reagents for 250 standard (5ml) assays or 1000 microtube (1.5-2 ml) assays

    Applications​
    • Estimate protein during protein purification, electrophoresis, cell biology, molecular biology, and other research applications.
    • Suitable for protein samples containing common laboratory agents, such as chelating agents (EDTA), detergents, amines (Tris), sugars and many other agents.
    • Suitable for samples containing chaotropic agents such as urea, thiourea, guanidine hydrochloride, guanidine thiocyanate, ammonium sulfate, drugs, antibiotics, cobalt, and numerous other agents and extraction buffers.
    • Suitable for determination of protein concentration in cellular fractions, tissue & cell lysates and chromatography purification fractions.
  • Reviews

G-Biosciences’ ML (Modified Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The ML Protein Assay is compatible with a wide variety of detergents used in protein research in addition to other common reagents such as EDTA and Tris (see tables 1 and 2). The assay is supplied with either traditional bovine serum albumin (BSA) protein standard, pre-diluted BSA protein standards, non animal protein standard or bovine ɣ globulin protein standard. 
 

A CL (Compatible Lowry) Protein Assay is available for samples containing reducing agents (such as dithiothreitol (DTT), ß-mercaptoethanol and TCEP) and a wide variety of commonly used laboratory agents that are known to interfere with Lowry’s protein assays (see tables 1 and 2).
 

Table-1: A selection of compounds and their maximum concentrations compatible with the ML and CL Protein Assay.

                     Reagent   ML Protein Assay   CL Protein Assay
Amino Acids Compatible -
Ammonium Sulfate 0.5M 40%
β-Mercaptoethanol X 5%, 15%*
Brij® 35 1% 1%
Calcium Chloride 0.05M -
CHAPS 1% 4%
CHAPSO 1% 1%
CTAB - 1M
Deoxycholate 1% -
Digitonin 0.3% -
DTT 0.001M 0.1M, 0.35M*
EDTA 0.025M 0.1M
Glycerol - 30%
Guanidine.HCl 0.4M 6M
Guanidine Thiocynate - 6M
HEPES - 0.1M
Hydrochloric Acid 0.5M -
Imidazole - 0.5M
Iodoacetamide - 15mM
Laemmli Buffer (w/5% β-Mercaptoethanol) - Compatible
NP-40 2% -
Octaethyleneglycol dodecyl ether 0.2% -
Octyl Glucoside 1% -
Phosphate Buffer - 0.2M
Sarcosyl - 1%
SDS 10% -
Sodium Azide 0.05% 0.1M
Sodium Chloride - 0.5M
Sodium Hydroxide 0.5M 2.5M
Sucrose - 30%
TCEP - 15mM
Thesit® 1% 2%
Thiourea - 2M
Tributylphosphine (TBP) 0.002M -
Tris (pH 8) 0.1M 0.5M
Triton® X-100 1% 3%
Triton® X-114 1% 3%
Tween® 20 1% 2%
Urea 4M 8M
Zwittergent® 3-12 - 1.5M

-, not tested; X, not compatible; *, two washes (optional).


 

Table 2: ML & CL Protein Assay are compatible with strong chaotropic extraction buffers

Buffer Composition
4M Urea, 1% SDS, 10mM EDTA, 0.8% β-Mercaptoethanol
6M Urea, 2M Thiourea, 4% CHAPS
6M Urea, 2M Thiourea, 4% NP-40
1% Sarcosyl, 0.8 β-Mercaptoethanol, 4M Guanidine Thiocyanate, 10mM EDTA
6M Urea, 2M Thiourea, 2% CHAPS, 2% ND SB 201
6M Urea, 2M Thiourea, 2% CHAPS, 2% SB 2 10
 

Features
  • Protein quantitation in the presence of detergents 
  • Quick 15 minute incubation
  • Increased color stability (color will not change more than 5% in 1 hour or 10% in 2 hours)
  • Linear response 0.2 – 1.5 mg/ml
  • One simple protocol without cumbersome sample preparation or buffer exchange steps
  • Convenient kit provides all necessary reagents for 250 standard (5ml) assays or 1000 microtube (1.5-2 ml) assays

Applications​
  • Estimate protein during protein purification, electrophoresis, cell biology, molecular biology, and other research applications.
  • Suitable for protein samples containing common laboratory agents, such as chelating agents (EDTA), detergents, amines (Tris), sugars and many other agents.
  • Suitable for samples containing chaotropic agents such as urea, thiourea, guanidine hydrochloride, guanidine thiocyanate, ammonium sulfate, drugs, antibiotics, cobalt, and numerous other agents and extraction buffers.
  • Suitable for determination of protein concentration in cellular fractions, tissue & cell lysates and chromatography purification fractions.

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