The Pearl™ IgG Purification kits are designed for the one step purification of IgG (Immunoglobulin G) antibodies from serum. The supplied Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulfate precipitation.
The Pearl™ IgG purification kits can purify IgG in <15 minutes, which is more rapid than the commonly used Protein A and Protein G resins. The performance of the Pearl™ IgG Purification Resin is comparable or better than the Protein A and Protein G resins.
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Species
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Pearl™ IgG Purification Resin
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Protein A
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Protein G
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Mouse
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++++
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++++
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++++
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Human
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++++
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++++
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++++
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Rat
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++++
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+
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++
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Hamster
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++++
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++
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++
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Guinea Pig
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++++
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++++
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++
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Rabbit
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++++
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++++
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+++
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Horse
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++++
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++
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++++
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Cow
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++
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++
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++++
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Pig
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++++
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+++
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++
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Sheep
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++
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+
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++
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Goat
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++++
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+
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++
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Chicken
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-
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-
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-
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The Pearl™ IgG Purification Kits are available in two formats:
Cat. # 786-798 is a spin format that is ideal for the rapid, small scale purification of IgG. The kit is supplied with 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Suitable for purifying up to 25mg IgG.
Cat. # 786-799 is supplied with 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer and is suitable for the isolation of IgG from ~100ml serum (~200mg IgG).
Also availlable is our Pearl™ Monoclonal IgG Purification Kit, which purifies monoclonal antibodies from ascites and cell culture supernatant.
Features
- Simple 1-step purification, eliminates Protein A & G binding and elution steps
- High recovery (>90%) & Purity (>80%)
- Purify IgG from sources not compatible with Protein A & G
Applications
- Purification of IgG (Immunoglobulin G) molecules
