Protein A (Recombinant)

Protein A is a cell-wall receptor which is present on cell wall of various starins of Staphyloccocus aureus. Protein A contains four high-affinity binding sites for interacting with the Fc region of immunoglobulin Gs of various species including mouse, rabbit and human. Protein A is used for making probes for detection of antibodies and for making affinity media for purification of antibodies.

Protein A (Recombinant) is recombinant protein A expressed in E. coli. It functions similarly as the native Protein A.

Protein A (Recombinant) can be conjugated with reporter molecules, fluorescent dyes, enzyme markers, biotin or colloidal gold for detection of antibodies by immunochemical assays including ELISA, western blotting or Immunohistochemistry.

Protein A (Recombinant) can be immobilized on a solid support such as agarose to purify monoclonal or polyclonal antibodies. 

Features:

• Source: E. coli.
• Molecular weight: ~47kDa; exact sequence as the native Protein A.
• Binds 3 IgG Isotypes.
• Purity: ≥ 95%, HPLC, Single major band.
• Form: Lyophilized powder.

Application(s):

• Protein A (Recombinant) conjugated to reporter molecules or fluourescent dyes or enzymemarkers can be used in detection of antibody by immunochemicals assays.
• Protein A (Recombinant)  immobilized on a solid support such as agarose is used to purify monoclonal or polyclonal antibodies. 

Image By E A S (Own work)