The CytoScan™ LDH Cytotoxicity Assay is a colorimetric method of assaying cellular cytotoxicity and is an alternative to 51Cr release cytotoxicity assays. The kit combines the advantages of reliability and simple evaluation, characteristic of radioisotope release assays, with the convenience of accuracy and avoidance of radioactivity. The CytoScan™ LDH Cytotoxicity Assay Kit can be used with different cell types for assaying cell mediated cytotoxicity as well as cytotoxicity mediated by chemicals and other test compounds.
The assay quantitatively measures the stable, cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium (INT)) into a red color formazan by diaphorase. The LDH activity is determined as NADH oxidation or INT reduction over a defined time period.
The resulting formazan absorbs maximally at 492nm and can be measured quantitatively at 490nm.
FEATURES
- Quantitatively measures LDH release
- Colorimetric assay
- Ideal for high throughput screening
APPLICATIONS
- For the detection of cell toxicity, death, viability or proliferation
- For cell free supernatants from cells in culture (adherent or suspension)
References
- Tomassian, T. et al (2011) J. Immunol. 187:2993
- Westwell-Roper, C. et al (2011) J. Immunol. 187:2755
- Sharma, S. et al (2011) International Journal of Toxicology. 30:358-366
- Litterst, C. et al (2009) Bio-Rad.
- Haslam, G. et al (2005) Anal. Biochem. 336: 187
- Tarnawski, A. (2005) Biochem. Biophys. Res. Comm. 333: 207
- Round, J. L et al (2005) J. Exp. Med. 201: 419
- Bose, C. et al (2005) Am. J. Physiol. Gastr. L. 289: G926
- Chen, A. and Xu, J. (2005) Am. J. Physiol. Gastr. L. 288: G447
Technical
- For a quick comparison of protein estimation assays, view our Protein Assay Selection Guide Table.
- For more information a wide range of bioassays, view our Bioassays Handbook: a detailed handbook & selection guide for apoptosis assays, cytotoxicity assays, SAM methyltransferase assays, protease assays, phosphatase assays, peroxide assays & ELISA.
- For more information on protein assays, view our Protein Assay Handbook: a handbook and selection guide for introduction to and selection of protein assays, including protein estimation assays, apoptosis assays, cytotoxicity assays, SAM methyltransferase assays, protease assays, phosphatase assays & peroxide assays.