We use cookies 🍪
We use cookies and other similar technologies to improve your browsing experience and the functionality of our site. Learn more in our Privacy Policy.
Cart0 item(s) - $0.00 0

  • Your shopping cart is empty!

DetergentOUT™ GBS10

DetergentOUT™ GBS10
0 reviews • Write a review
DetergentOUT™ GBS10
DetergentOUT™ GBS10
Catalog Description Size Price (USD) Qty
786-154DetergentOUT™ GB-S10-12510 columns$77.00
786-155DetergentOUT™ GB-S10-80010 columns$89.00
786-156DetergentOUT™ GB-S10-300010 columns$233.00
786-157DetergentOUT™ GB-S10-500010 columns$322.00
786-159DetergentOUT™ GB-S1010ml resin$362.00
786-998DetergentOUT™ GB-S10 Spin Plates2 Plates$622.00
Add to Cart
  Request Quote
0 reviews • Write a review
Add to Cart
Buy Now Question?
Add to Wish List
  • Description

    Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins, and suppress peptide ionization when analyzed by mass spectrometry.

     

    DetergentOUT™ GBS10 features a research grade detergent binding resin that has a high affinity for most detergents and a low affinity for proteins and peptides. DetergentOUT™ GBS10 is ideal for detergent removal prior to ELISA, IEF, protease digestion of proteins, and peptide ionization when analyzed by mass spectrometry.

     

    DetergentOUT™ GBS10 is available in four spin format column sizes or as resin alone.  The column specifications are:

    Cat. # Description Sample Size (µl) Resin Volume (µl)
    786-154 DetergentOUT™ GBS-10-125 10-30 125
    786-155 DetergentOUT™ GBS-10-800 30-200 800
    786-156 DetergentOUT™ GBS-10-3000 200-750 3,000
    786-157 DetergentOUT™ GBS-10-5000 500-1,250 5,000

     

    Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss (Table below) for downstream analysis by mass spectrometry (Figure 1 & 2) and other techniques.

     

    Also available in 96-well spin plate formats for processing up to 96 samples.

     

    The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF and LC-MS/MS (see references). The use of the DetergentOUT™ GBS10 columns significantly increased the number of peptide spectra detected (Figure 1 & 2).

     

    In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, with 6 milligrams SDS (Figure 3) and 14 milligrams Triton® X-100 for every milliliter of settled resin.

     

     Detergent % Removed BSA Phosphorylase B  Cytochrome C  E.coli Lysate
     Triton X-100, 2%  >99  >90  >91  >92  >93
     Triton X-114, 2%  >96  >99  >98 >97  >91
     Nonidet P-40, 1%  >96  >93  >95  >91  >91
     Brij 35, 1%  >99  >98  >99  >97  >91
     SDS, 2.5%  >99  >96  >97  >92  >90
     Sodium deoxycholare, 5%  >99  >99  >99  >98  >95
     CHAPS, 3%  >99  >92  >95  >92  >91
     Octyl glucoside, 5%  >99  >93  >95  >96  >91
     Lauryl maltoside, 1%  >97  >99  >99  >99  >91
     Tween 20*, 0.25%  >98  >86  >85  >89  >85
     Tween 80*, 0.13%  >85  >83  >81  >80  >81

    Detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10

     

     


    Features

    • Easy-to-use, spin-format columns
    • Rapid removal of free detergents
    • Suitable for anionic, non-ionic and zwitterionic detergents
    • Minimal sample loss
    • Available for sample volumes ranging from 10µl to 1,250µl

     


    Applications

    • Detergent removal from protein and peptide solutions
    • Detection of peptide fragments by Mass spectrometry
    • Enhances Mass spectrometry Spectra
    • Ideal for Mass spectrometry sample preparation and prior to downstream analysis and applications

     

     

  • Reviews

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins, and suppress peptide ionization when analyzed by mass spectrometry.

 

DetergentOUT™ GBS10 features a research grade detergent binding resin that has a high affinity for most detergents and a low affinity for proteins and peptides. DetergentOUT™ GBS10 is ideal for detergent removal prior to ELISA, IEF, protease digestion of proteins, and peptide ionization when analyzed by mass spectrometry.

 

DetergentOUT™ GBS10 is available in four spin format column sizes or as resin alone.  The column specifications are:

Cat. # Description Sample Size (µl) Resin Volume (µl)
786-154 DetergentOUT™ GBS-10-125 10-30 125
786-155 DetergentOUT™ GBS-10-800 30-200 800
786-156 DetergentOUT™ GBS-10-3000 200-750 3,000
786-157 DetergentOUT™ GBS-10-5000 500-1,250 5,000

 

Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss (Table below) for downstream analysis by mass spectrometry (Figure 1 & 2) and other techniques.

 

Also available in 96-well spin plate formats for processing up to 96 samples.

 

The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF and LC-MS/MS (see references). The use of the DetergentOUT™ GBS10 columns significantly increased the number of peptide spectra detected (Figure 1 & 2).

 

In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, with 6 milligrams SDS (Figure 3) and 14 milligrams Triton® X-100 for every milliliter of settled resin.

 

 Detergent % Removed BSA Phosphorylase B  Cytochrome C  E.coli Lysate
 Triton X-100, 2%  >99  >90  >91  >92  >93
 Triton X-114, 2%  >96  >99  >98 >97  >91
 Nonidet P-40, 1%  >96  >93  >95  >91  >91
 Brij 35, 1%  >99  >98  >99  >97  >91
 SDS, 2.5%  >99  >96  >97  >92  >90
 Sodium deoxycholare, 5%  >99  >99  >99  >98  >95
 CHAPS, 3%  >99  >92  >95  >92  >91
 Octyl glucoside, 5%  >99  >93  >95  >96  >91
 Lauryl maltoside, 1%  >97  >99  >99  >99  >91
 Tween 20*, 0.25%  >98  >86  >85  >89  >85
 Tween 80*, 0.13%  >85  >83  >81  >80  >81

Detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10

 

 


Features

  • Easy-to-use, spin-format columns
  • Rapid removal of free detergents
  • Suitable for anionic, non-ionic and zwitterionic detergents
  • Minimal sample loss
  • Available for sample volumes ranging from 10µl to 1,250µl

 


Applications

  • Detergent removal from protein and peptide solutions
  • Detection of peptide fragments by Mass spectrometry
  • Enhances Mass spectrometry Spectra
  • Ideal for Mass spectrometry sample preparation and prior to downstream analysis and applications

 

 

Be the first to write a review for this product.

Write a review

Note: HTML is not translated!
Bad           Good
Captcha