Agarose I (All purpose agarose)
Agarose I (All purpose agarose)
Agarose I (All purpose agarose)
| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| RC-005 | Agarose I (All Purpose, Electrophoresis Matrix (Low EEO)) | 25g | $82.00 | |
| RC-006 | Agarose I (All Purpose, Electrophoresis Matrix (Low EEO)) | 100g | $236.00 | |
| RC-122 | Agarose I (All Purpose, Electrophoresis Matrix (Low EEO)) | 5g | $23.00 | |
| RC-149 | Agarose I (All Purpose, Electrophoresis Matrix (Low EEO)) | 500g | $1,051.00 |
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Description
Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).Specifications
- Moisture: ≤ 7%
- Ash: 4%
- EEO: 0.05-0.13
- Sulfate: ≤ 0.1%
- Clarity 1.5% (NTU): ≤ 3
- Gel Strength 1% (g/cm2): ≥ 1200
- Gel Strength 1.5% (g/cm2): ≥ 2500
- Gelling Temperature 1.5% (ºC): 36 ± 1.5
- Melting Temperature 1.5% (ºC): 88 ± 1.5
- DNase/ RNase Activity: None Detected
- DNA Resolution ≥1000bp: Finely resolved
- Gel background: Very low
Appropriate Agarose ConcentrationsGEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER 0.6 1,000-20,000bp 1,000-15,000bp 0.8 500-12,000bp 500-10,000bp 1.0 300-8,000bp 250-7,000bp 1.2 200-6,000bp 200-5,000bp 1.5 100-3,500bp 100-3,000bp 2.0 50-2,000bp 50-2,000bp
Features-
Extraordinary mechanical resistance for more reliable and easier handling
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Greater thermal stability due to high hysteresis (difference between gelling and melting temp)
-
High transparency and visibility
-
Easy preparation
Applications-
Analytical nucleic acid electrophoresis
-
Preparative nucleic acid electrophoresis
-
Blotting
-
Protein electrophoresis such as radial immunodiffusion
- Reviews
Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility. Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).
Specifications
- Moisture: ≤ 7%
- Ash: 4%
- EEO: 0.05-0.13
- Sulfate: ≤ 0.1%
- Clarity 1.5% (NTU): ≤ 3
- Gel Strength 1% (g/cm2): ≥ 1200
- Gel Strength 1.5% (g/cm2): ≥ 2500
- Gelling Temperature 1.5% (ºC): 36 ± 1.5
- Melting Temperature 1.5% (ºC): 88 ± 1.5
- DNase/ RNase Activity: None Detected
- DNA Resolution ≥1000bp: Finely resolved
- Gel background: Very low
Appropriate Agarose Concentrations
| GEL CONCENTRATION (%) | 1X TAE BUFFER | 1X TBE BUFFER |
| 0.6 | 1,000-20,000bp | 1,000-15,000bp |
| 0.8 | 500-12,000bp | 500-10,000bp |
| 1.0 | 300-8,000bp | 250-7,000bp |
| 1.2 | 200-6,000bp | 200-5,000bp |
| 1.5 | 100-3,500bp | 100-3,000bp |
| 2.0 | 50-2,000bp | 50-2,000bp |
Features
-
Extraordinary mechanical resistance for more reliable and easier handling
-
Greater thermal stability due to high hysteresis (difference between gelling and melting temp)
-
High transparency and visibility
-
Easy preparation
Applications
-
Analytical nucleic acid electrophoresis
-
Preparative nucleic acid electrophoresis
-
Blotting
-
Protein electrophoresis such as radial immunodiffusion