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Agarose I (All purpose agarose) (7 Citations)

Agarose I has low electroendosmosis (EEO) and high electrophoresis mobility.  Suitable for nucleic acid analytical and preparative electrophoresis; blotting; and protein electrophoresis (i.e. Radial Immunodiffusion).
 
Specifications
  • Moisture: ≤ 7%
  • Ash: 4%
  • EEO: 0.05-0.13
  • Sulfate: ≤ 0.1%
  • Clarity 1.5% (NTU): ≤ 3
  • Gel Strength 1% (g/cm2): ≥ 1200
  • Gel Strength 1.5% (g/cm2): ≥ 2500
  • Gelling Temperature 1.5% (ºC): 36 ± 1.5
  • Melting Temperature 1.5% (ºC): 88 ± 1.5
  • DNase/ RNase Activity: None Detected
  • DNA Resolution ≥1000bp: Finely resolved
  • Gel background: Very low
 
Appropriate Agarose Concentrations
GEL CONCENTRATION (%) 1X TAE BUFFER 1X TBE BUFFER
0.6 1,000-20,000bp 1,000-15,000bp
0.8 500-12,000bp 500-10,000bp
1.0 300-8,000bp 250-7,000bp
1.2 200-6,000bp 200-5,000bp
1.5 100-3,500bp 100-3,000bp
2.0 50-2,000bp 50-2,000bp

 


Features

  • Extraordinary mechanical resistance for more reliable and easier handling
  • Greater thermal stability due to high hysteresis (difference between gelling and melting temp)
  • High transparency and visibility
  • Easy preparation

 


Applications

  • Analytical nucleic acid electrophoresis
  • Preparative nucleic acid electrophoresis
  • Blotting
  • Protein electrophoresis such as radial immunodiffusion

 

 

Material Safety Data Sheet
RC-005
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RC-006
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RC-122
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RC-149
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Technical Literature
Molecular Biology Handbook  A guide to our products for DNA and RNA.
Certificate Of Analysis
RC-005
090712
111207
122408
131207
131906
132404
RC-006
120507
122408
090712
100504
100802
101105
101801
101902
102101
102608
102709
111207
RC-122
090911
092411
101105
102202
110203
110411
131107
131906
132404
RC-149
091510
100504
101902
102203
102709
102912
110411
111207
122408
132404

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