Pearl™ IgG Purification Kits

The Pearl™ IgG Purification kits are designed for the one step purification of IgG (Immunoglobulin G) antibodies from serum. The supplied Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulfate precipitation.

The Pearl™ IgG purification kits can purify IgG in <15 minutes, which is more rapid than the commonly used Protein A and Protein G resins. The performance of the Pearl™ IgG Purification Resin is comparable or better than the Protein A and Protein G resins.

The Pearl™ IgG Purification Kits are available in two formats:

Cat. # 786-798 is a spin format that is ideal for the rapid, small scale purification of IgG. The kit is supplied with 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns.  Suitable for purifying up to 25mg IgG.

Cat. # 786-799 is supplied with 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer and is suitable for the isolation of IgG from ~100ml serum (~200mg IgG).

Also availlable is our Pearl™ Monoclonal IgG Purification Kit, which purifies monoclonal antibodies from ascites and cell culture supernatant.

Features

• Simple 1-step purification, eliminates Protein A & G binding and elution steps
• High recovery (>90%) & Purity (>80%)
• Purify IgG from sources not compatible with Protein A & G

Applications

• Purification of IgG (Immunoglobulin G) molecules