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ProteCEASE™

ProteCEASE™
1 reviews • Write a review
ProteCEASE™
ProteCEASE™
Catalog Description Size Price (USD) Qty
786-326ProteCEASE™-50, EDTA free10 Vials$307.00
786-326TProteCEASE™-50, EDTA free1 Vial$80.00
786-327ProteCEASE™-50, EDTA free20 Vials$603.00
786-328ProteCEASE™-100, EDTA free10 Vials$599.00
786-334ProteCEASE™-50, plus EDTA10 Vials$307.00
786-335ProteCEASE™-50, plus EDTA20 Vials$603.00
786-336ProteCEASE™-100, plus EDTA10 Vials$599.00
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  • Description

     

    ProteCEASE™ is a dry format version of our ProteaseArrest™ for large scale preparative applications and for those who prefer reconstitution prior to use.

     

    ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

     

    The EDTA-free ProteCEASE™ will maintain activity of proteins dependent on divalent cations and will not inhibit the purification of proteins with immobilized metal affinity chromatography (IMAC).

     

    ProteCEASE™ has been specifically developed for large scale preparative applications and is available in two vial sizes: ProteCEASE™-50 and ProteCEASE™-100, for 50 and 100 milliters of lysis buffer, respectively.

     

    ProteCEASE™-50 is available in packs of 10 or 20 vials for a total volume of either 500 milliters or 1 liter.

     

    ProteCEASE™-100 is available in packs of 10 for 1 liter total volume.

     

     


    Features

    • High protease inhibition: >95% in mouse pancreas lysate
    • Inhibits a wide variety of proteases, including cysteine, serine and metallo-proteases
    • Available with or without EDTA
    • Performs with a wide range of samples, including animals, plants, yeast, bacteria and fungal lysates

     


    Applications

    • For large scale preparative protein purifications
    • Inhibition of protease activity in protein preparations
    • Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays and other applications

     

     

  • Reviews

 

ProteCEASE™ is a dry format version of our ProteaseArrest™ for large scale preparative applications and for those who prefer reconstitution prior to use.

 

ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

 

The EDTA-free ProteCEASE™ will maintain activity of proteins dependent on divalent cations and will not inhibit the purification of proteins with immobilized metal affinity chromatography (IMAC).

 

ProteCEASE™ has been specifically developed for large scale preparative applications and is available in two vial sizes: ProteCEASE™-50 and ProteCEASE™-100, for 50 and 100 milliters of lysis buffer, respectively.

 

ProteCEASE™-50 is available in packs of 10 or 20 vials for a total volume of either 500 milliters or 1 liter.

 

ProteCEASE™-100 is available in packs of 10 for 1 liter total volume.

 

 


Features

  • High protease inhibition: >95% in mouse pancreas lysate
  • Inhibits a wide variety of proteases, including cysteine, serine and metallo-proteases
  • Available with or without EDTA
  • Performs with a wide range of samples, including animals, plants, yeast, bacteria and fungal lysates

 


Applications

  • For large scale preparative protein purifications
  • Inhibition of protease activity in protein preparations
  • Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays and other applications

 

 

William Plaxton 03/05/2019

ProteCEASE-100 outperforms Sigma's Protease Inhibitor Cocktail for plant cell and tissue extracts (Cat. # P9599). Although Sigma’s website claims that this PIC has been ‘Optimized and tested for use with plant tissue and cell extracts, it has proven incapable of suppressing unwanted partial proteolysis of plant- and bacterial-type phosphoenolpyruvate (PEP) carboxylases (PEPCs) from developing castor beans, or glutamine synthetase from leaves of Medicago trunculata. In our experience, the ProtCEASE-100 PIC marketed by G-Biosciences has been quite effective at inhibiting unwanted proteolysis of native enzymes that we have extracted and purified from various plant sources. For example, ProtCEASE-100 (but not several other commercially available PICs, including Sigma’s aforementioned ‘plant-specific’ PIC) suppressed proteolytic cleavage of 118 kDa bacterial-type PEPC polypeptides. See Plaxton, W.C. (2019) Plant & Cell Physiology. 60:715-724 for experimental details.

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