Site Catalog Number   Welcome Guest | Register | Login Items:0 View Cart CheckOut My Account   Protease-ArrestTM Kills Proteases On Contact For A Complete Inhibition of Protease Activities in extracts from animal cells and tissues, plants, yeast or bacteria. Protease-ArrestTM contains agents to inhibit serine, cysteine, metalloproteases as well as calpains proteases. Protease-ArrestTM contains both irreversible and reversible protease inhibitors and an optional EDTA solution is provided for inhibition of metalloproteases. Due to the optimized concentration of the various inhibitors, Protease-ArrestTM shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during preparation of samples and protein purification from animal tissues, plants, yeast and bacteria.  Since some proteins require divalent cations like Ca2+ , Mg2+, or Mn2+ , for their biological activity, the presence of EDTA may be detrimental to protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. Protease-Arrest is therefore supplied with an optional EDTA solution and may be added in the extraction buffer or lysate as needed. Ready-to-use solution-simply add an appropriate volume of Protease-ArrestTM in protein extract or extraction buffer to inhibit protease activity. Supplied as 100X solutions. Simple to use, add 10-20ml Protease-ArrestTM/ml in extraction buffer to inhibit protease activity. FEATURES A broad spectrum protease inhibitor cocktail. Protease-ArrestTM at 1X concentration in extraction buffer at pH 7-8 inhibits ~ 95% of protease activities (i.e. Mouse Pancreas Extract, 0.5 mg /ml protein). Supplied as 100X solution. APPLICATIONS Inhibition of protease activity in protein preparations. Protection of proteins from proteolysis in such applications as electrophoresis, purification, storage, assays, and other applications. Catalog # Description Size Shipping Condition Price   786-108 Protease-Arrest™[100X], with EDTA, 2 ml 2 ml Ambient US $129.00 Books | Specials | Order | Register| Contact | MSDS | Employment | Branches and Distributors | Site Map © Geno Technology, Inc. All rights reserved.