Western ReProbe™

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots.

Western ReProbe™ breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new proteins.

Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).

Western ReProbe™ is not recommended for stripping color-producing western blots that use substrates such as TMB, chloronapthol and DAB.

Western ReProbe™ is supplied as a 5X concentrated solution. Each 100 milliliters is sufficient for 25 to 30 standard size (7.5cm x 8.5cm) western blots.

For high affinity antibodies, we recommend our Western ReProbe™ PLUS.

Features

• Simply incubate the blot in Western ReProbe™ at room temp & wash
• No boiling, denaturants or SDS required
• Repeated analysis of Western blots with multiple probes
• Re-analysis & correction of unsatisfactory Western blots
• Conservation of hard-to-obtain test samples & reagents
• Time saving in not rerunning gels & transfer processes

Applications

• Screen same blot with different antibodies
• Reprobe for housekeeping proteins
• Compare phosphorylated & total protein on the same blot