Western ReProbe™

Western ReProbe™

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse western blots.

Western ReProbe™ breaks the antigen-antibody binding affinity without using denaturants, SDS or boiling. The membrane-bound protein is retained on the membrane, and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new proteins.

Removal of antibodies also removes corresponding chemiluminescent or radio-isotopic signals from the blots. The stripped blots can then be probed with new probes (see figure 1).

Western ReProbe™ is not recommended for stripping color-producing western blots that use substrates such as TMB, chloronapthol and DAB.

Western ReProbe™ is supplied as a 5X concentrated solution. Each 100 milliliters is sufficient for 25 to 30 standard size (7.5cm x 8.5cm) western blots.

For high affinity antibodies, we recommend our Western ReProbe™ PLUS.


Features

  • Simply incubate the blot in Western ReProbe™ at room temp & wash
  • No boiling, denaturants or SDS required
  • Repeated analysis of Western blots with multiple probes
  • Re-analysis & correction of unsatisfactory Western blots
  • Conservation of hard-to-obtain test samples & reagents
  • Time saving in not rerunning gels & transfer processes

Applications

  • Screen same blot with different antibodies
  • Reprobe for housekeeping proteins
  • Compare phosphorylated & total protein on the same blot

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Protocol
786-119
786-305
786-306
786-818
Material Safety Data Sheet
786-119
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Dutch
English_UK
French
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Polish
786-305
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-306
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
786-818
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Technical Literature
Multiple Probing of Western Blots
Western Blotting Handbook From protein electrophoresis and Western transfer to blocking, probing and detection of membrane immobilized proteins.
Catalog Description Size Price(USD) Qty
786-119
Western ReProbe™ [5X] 100ml
$113.00
786-305
Western ReProbe™ [5X] 500ml
$303.00
786-306
Western ReProbe™ [5X] 1L
$464.00
786-818
Western ReProbe™ [5X] 1 Gallon
$1,390.00