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1-Fluoro-2,4-dinitrobenzene [DNFB]

1-Fluoro-2-4-dinitrobenzene-DNFB
Catalog Description Size Price(USD) Qty
786-1667
1-Fluoro-2,4-dinitrobenzene [DNFB] 5 g
$35.00

1-Fluoro-2,4-dinitrobenzene [DNFB]

1-Fluoro-2,4-dinitrobenzene [DNFB], commonly known as Sanger’s reagent is a chemical amino acid modifier that reacts with N-terminal amino acid of polypeptides. In Sanger reaction DNFB is used to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary amines.

 

Features

  • Molecular Formula:C6H3FN2O4
  • Molecular Weight: 186.1
  • CAS Number: 70-34-8
  • Melting point: 26°C
  • Density: 1.48/ml
  • Molarity: 7.95 M
  • Solubility: Soluble in chloroform at 100 mg/ml. Also soluble in benzene, ether and propylene glycol.
  • Storage/Stability: The half life at 15°C in Tris buffer is 56.7 hours at pH8.03 and 6.5 hours at pH9.53

 

Application(s)

  • Used in Sanger reaction to form dinitrophenyl derivatives for end group determination of proteins and to derivatize primary amines.
  • Depending upon its property of reaction with N-terminal amino acid of polypeptides DNFB can also be used to inhibit activity of some enzymes.
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