Alamar Blue Cell Viability Assay (6 Citations)
AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye in the Alamar blue assay incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes color in response to the chemical reduction of growth medium due to cell growth.
The alamarBlue (resazurin) dye in its oxidized form is blue in color and non-fluorescent. In alamarBlue assay the growing cells cause a chemical reduction of the alamarBlue dye from non-fluorescent blue to red fluorescent. The continued growth of viable cells maintain a reducing environment (fluorescent, red) and inhibition of growth maintains an oxidized environment (non-fluorescent, blue), which can be detected using a fluorescence or absorbance detector.
- High sensitivity and linerarity
- Non-toxic to cells
- No cell lysis
- Flexible and versatile
- Monitor cell proliferation and cell viability for in vitro cytotoxicity studies (e.g., effects of environmental toxins, cytokines, drugs, siRNA/microRNA)
- Monitor cell viability in real time for 3D tissue engineering applications
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- Kamiloglu, S. et al (2020) Food Frontiers. DOI: 10.1002/fft2.44
- Peng, JY. (2019) Evaluation of antiviral activity of Bacillus licheniformis-fermented products against porcine epidemic diarrhea virus. AMB EXPRESS. https://doi.org/10.1186/s13568-019-0916-0
- Hsueh, CS. et al (2019) Role of nuclear factor-kappa B in feline injection site sarcoma. Role of nuclear factor-kappa B in feline injection site sarcoma.
- Jyotsana, N. et al (2019) Minimal dosing of leukocyte targeting TRAIL decreasestriple-negative breast cancer metastasis followingtumor resection. Sci Adv.DOI: 10.1126/sciadv.aaw4197.
- Peng, JY. Evaluation of antiviral activity of Bacillus licheniformis-fermented products against porcine epidemic diarrhea virus. AMB EXPRESS. 9:191.
- Zipperer, A. and Kretschmer, D. (2016) Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents. Methods in Mol. Biol. 1520:107