CytoScan™ WST-1 Cell Proliferation Assay is a sensitive and accurate assay for cell proliferation and cytotoxicity. The assay is highly convenient as it is performed in a single cell tissue culture well and requires no washing, harvesting or solubilization of cells.
Adherent or suspension cells are cultured in a microplate and then incubated with WST-1 and the assay is monitored with a spectrophotometer. The assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow colored formazan is measured at 420-480nm (optimal at 440nm) and is directly correlated to cell number.
The kit components are sufficient for performing up to 500 or 2,000 assays.
Features
-
Uses WST-1, a high sensitivity tetrazolium salt.
-
Colorimetric assay.
-
Adherent or suspension cells.
-
No washing, harvesting or solubilization required.
Applications
-
For the detection of cell toxicity, death, viability or proliferation.
-
Ideal for high throughput screening.
