Immobilized Papain

Immobilized Papain

Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region.  This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain.  The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis. 

 

The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography. 

 

Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.

 

Click here to request bulk or custom sizes.
 

 


Features

  • Generate Fc and Fab from IgG
  • Eliminates contamination with papain enzyme
  • Can be used in virtually all scenarios using free papain

 

 

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Protocol
786-790
786-812
Material Safety Data Sheet
786-790
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786-812
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Technical Literature
Antibody Handbook
Protease & Phosphatase Inhibitors & Proteases Handbook  A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays
Catalog Description Size Price(USD) Qty
786-790
Immobilized Papain 5ml Resin
$194.00
786-812
Immobilized Papain 25ml resin
$920.00