Papain is a cysteine protease enzyme (EC 126.96.36.199) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
- Generate Fc and Fab from IgG
- Eliminates contamination with papain enzyme
- Can be used in virtually all scenarios using free papain
|Material Safety Data Sheet|
|Protease & Phosphatase Inhibitors & Proteases Handbook||A handbook & selection guide for inhibitors of protease & phosphatases & for proteases & assays|
- Offenbacher, A.R. et al (2017) Hydrogen-deuterium exchange reveals long-range dynamical allostery in soybean lipoxygenase. J. Biol. Chem.DOI: 10.1074/jbc.M117.817197.
- Tolbert, W. D. et al (2016) Paring Down HIV Env: Design and Crystal Structure of a Stabilized Inner Domain of HIV-1 gp120 Displaying a Major ADCC Target of the A32 Region.Journal/ Year/ Vol #: Structure/2016/24
|Immobilized Papain||5ml Resin||
|Immobilized Papain||25ml resin||