BCA Protein Assay for Dilute Samples

The Micro BCA Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the BCA Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu²⁺ ions, in a temperature dependent reaction, from the copper solution to Cu⁺. The amount of Cu²⁺ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid chelate with each Cu⁺ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562nm. The assay uses concentrated solutions and extended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations. The Micro BCA Protein Assay is suitable for quantifying protein solutions in 500 x 1ml assays or in >3,300 micro-well assays. The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

Features

• Sensitive, colorimetric assay for dilute protein samples
• Linear range: 0.5-20µg/ml
• Compatible with most non-ionic and ionic detergents

Applications

• For dilute protein samples (0.5-20µg/ml)

For information on our regular Bicinchoninic Acid (BCA) Protein Assay, click here.  For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.