Citraconic Anhydride

Citraconic Anhydride

Citraconic anhydride reacts with primary amines and blocks them by creating an amide linkage and a terminal carboxylate.

 

The linkage is stable at neutral to alkaline pH (pH >7) and at acidic conditions (pH 4) the amide linkage is rapidly hydrolyzed to release the citraconic acid and free the amines. The block by citraconic anhydride can also be reveresed by treatment with hydroxylamine.  This property makes citraconic anhydride a very useful tool for blocking free amines in proteins and other biomolecules.
 


Features

  • Reversible blocking of primary amines
  • Reactive towards primary amines
  • Synonym: 2-methylmaleic anhydride
  • Empirical formula:C5H4O3
  • CAS # 616-02-4
  • Molecular weight: 112.08
  • Form: Colorless to slight yellow, clear liquid

 



Applications

  • Temporarily block amines to allow derivatization of other parts of the molecule.
  • Block removed by shifting to acidic conditions (pH3-4) or treatment with hydroxylamine

 

 

Write a review

    Bad           Good

Protocol
786-389
Material Safety Data Sheet
786-389
English_US
Danish
Dutch
English_UK
French
German
Norwegian
Portuguese
Swedish
Polish
Catalog Description Size Price(USD) Qty
786-389
Citraconic Anhydride 10g
$24.00