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Ellman’s Reagent

Ellman’s Reagent
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Ellman’s Reagent
Ellman’s Reagent
Catalog Description Size Price (USD) Qty
BC87Ellman’s Reagent5g$137.00
RC-483Ellman's Reagent, 5-5-Dithiobis (2-nitrobenzoic-acid)100g$1,655.00
RC-484Ellman's Reagent, 5-5-Dithiobis (2-nitrobenzoic-acid)25g$469.00
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  • Description
    5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)
     
    Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
     
    Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
     
    Synonyms: DTNB; 5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide
     
    MOLECULAR BIOLOGY GRADE
     
    PubChem Chemical ID: 6254
     
    Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
     
    Store at 2-8°C and protect from light and humidity.

     


    Features

    • Highly stable and water soluble
    • Quick and simple assay
    • CAS#: 69-78-3
    • Molecular Formula:  C14H8N2O8S2
    • Molecular Weight: 396.36 g/mol 

     


    Applications

    • Quantification of free sulfhydryl residues

     

     

  • Reviews
5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)
 
Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
 
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
 
Synonyms: DTNB; 5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide
 
MOLECULAR BIOLOGY GRADE
 
PubChem Chemical ID: 6254
 
Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
 
Store at 2-8°C and protect from light and humidity.

 


Features

  • Highly stable and water soluble
  • Quick and simple assay
  • CAS#: 69-78-3
  • Molecular Formula:  C14H8N2O8S2
  • Molecular Weight: 396.36 g/mol 

 


Applications

  • Quantification of free sulfhydryl residues

 

 

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