Direct ELISA of Bacterial alpha Amylase (Lab 6E)

This kit is designed to teach 8 groups of four students the principles of ELISA (Enzyme Linked ImmunoSorbent Assays).

The kit contains the reagents to allow students to:

• Conduct a serial dilution of a stock amylase solution to create standards of known concentration
• Bind known and unknown amylase samples to ELISA wells
• Wash and recognize amylase protein with an enzyme-labeled antibody
• Detect amylase-bound, enzyme-labeled antibody with a colored substrate
• Quantify the amount of amylase protein in unknown samples by using a standard curve of known amylase concentrations

Features

• For 8 groups of 4 students 
• Requires micropipettes and tips
• Optional requirement for a plate reader

Applications

• HS-LS1: From Molecules to Organisms: Structures and Processes
• LS1.A: Structure and Function