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Confirming the Amylase gene using PCR (Lab 13H)

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)

 

The kit contains the reagents to allow students to:

  • Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates
  • Set up a PCR negative control 
  • Carry out the PCR reaction
  • Load, separate, and visualize PCR DNA products on an agarose gel
  • Confirm a suspected origin of the amylase gene in nature

 


Features

  • For 8 groups of 4 students 
  • Requires thermocycler/ PCR machine, agarose electrophoresis equipment including UV transilluminator (light box) and micropipettes and tips

 


Applications

  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

 

 

Protocol
BTNM-13H
Material Safety Data Sheet
BTNM-13H
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