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Restriction Digestion to Verify the p-Amylase Plasmid (Lab 8b)

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to:

  • Set up restriction digests of the p-Amylase plasmid with two enzymes
  • Set up positive and negative controls for the restriction digestion
  • Load and separate DNA samples on an agarose gel
  • Visualize digests of the plasmid on an agarose gel
  • Construct the restriction digestion map of the plasmid

 

Features

  • For 8 groups of 4 students 
  • Requires agarose electrophoresis equipment including UV transilluminator (light box)
  • Micropipettes and tips

 

Applications

  • HS-LS1: From Molecules to Organisms: Structures and Processes
  • LS1.A: Structure and Function

 

 

Protocol
BTNM-8B
Material Safety Data Sheet
BTNM-8B
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