This kit is designed to teach 8 groups of four students the principles of Ion Exchange Chromatography and protein purification. The kit contains the reagents to allow students to:
- Equilibrate a DEAE agarose column
- Load a protein sample on the column (amylase, lysozyme or a mixture of both)
- Wash the unbound protein sample from the column
- Bind and then elute the bound protein sample from the column with a salt buffer
- Measure the amount of eluted protein with a Bradford protein assay
- Generate an elution profile determining which protein blinded to the column and which flowed through
- Give evidence that the two proteins were separated by the chromatography technology
Features
- For 8 groups of 4 students
- Requires micropipettes and tips
- Optional requirement for a spectrophotometer
Applications
- HS-LS1: From Molecules to Organisms: Structures and Processes
- LS1.A: Structure and Function
