Confirming the Amylase gene using PCR (Lab 13H)

This kit is designed to teach 8 groups of four students the principles of the polymerase chain reaction (PCR)

The kit contains the reagents to allow students to:

• Set up multiple PCR reactions using pAmylase plasmid DNA and bacterial genomic DNA templates
• Set up a PCR negative control 
• Carry out the PCR reaction
• Load, separate, and visualize PCR DNA products on an agarose gel
• Confirm a suspected origin of the amylase gene in nature

Features

• For 8 groups of 4 students 
• Requires thermocycler/ PCR machine, agarose electrophoresis equipment including UV transilluminator (light box) and micropipettes and tips

Applications

• HS-LS1: From Molecules to Organisms: Structures and Processes
• LS1.A: Structure and Function