Mutation Detection and Analysis
A PCR Based Laboratory Research Experiment. A major challenge for molecular biologists and genetic engineers is to easily detect and analyze genetic mutations that occur naturally, causing diseases, or during genetic engineering or cloning, whether deliberate or accidental.
Students learn about different types of genetic mutations, including substitutions, deletions and point mutations and about various techniques used to detect genetic mutations.
The kit contains all the reagents to screen simulated clinical samples for a mutant gene, using both the polymerase chain reaction (PCR) and restriction digestion mapping.
Students conduct a simple clinical diagnostic experiment in order to identify a diseased patient.
Supplied with components needed for hands-on experimentation for six workstations of 4-5 students or 24-30 students. Supplied with Teacher’s Guide and separate Student’s Guides.
- Understand detection and analysis of genetic mutations.
- Carry out a polymerase chain reaction (PCR) to amplify DNA source.
- Screen amplified DNA using restriction mapping with restriction enzymes.
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