Restriction Digestion to Verify the p-Amylase Plasmid (Lab 8b)

This kit is designed to teach 8 groups of four students the principles of restriction digestion and agarose electrophoresis. The kit contains the reagents to allow students to:

• Set up restriction digests of the p-Amylase plasmid with two enzymes
• Set up positive and negative controls for the restriction digestion
• Load and separate DNA samples on an agarose gel
• Visualize digests of the plasmid on an agarose gel
• Construct the restriction digestion map of the plasmid

Features

• For 8 groups of 4 students 
• Requires agarose electrophoresis equipment including UV transilluminator (light box)
• Micropipettes and tips

Applications

• HS-LS1: From Molecules to Organisms: Structures and Processes
• LS1.A: Structure and Function