Ellman’s Reagent

Catalog	Description	Size	Price(USD)	Qty
BC87	Ellman’s Reagent	5g	$101.00
RC-483	Ellman's Reagent, 5-5-Dithiobis(2-nitrobenzoic-acid)	100 G	$1,229.00
RC-484	Ellman's Reagent, 5-5-Dithiobis(2-nitrobenzoic-acid)	25 G	$379.00

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Ellman’s Reagent

5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)

Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.

Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.

Synonyms: DTNB; 5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide

MOLECULAR BIOLOGY GRADE

PubChem Chemical ID: 6254

Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.

Store at 2-8°C and protect from light and humidity.

Features

Highly stable and water soluble
Quick and simple assay
CAS#: 69-78-3
Molecular Formula: C₁₄H₈N₂O₈S₂
Molecular Weight: 396.36 g/mol

Applications

Quantification of free sulfhydryl residues

Protocol
BC87

Material Safety Data Sheet
BC87	English_US Danish Dutch English_UK French German Norwegian Portuguese Swedish Polish
RC-483	English_US Danish Dutch English_UK French German Norwegian Portuguese Swedish Polish
RC-484	English_US Danish Dutch English_UK French German Norwegian Portuguese Swedish Polish