Ellman’s Reagent

Ellman’s Reagent
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Ellman’s Reagent
5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)
 
Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
 
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
 
Synonyms: DTNB; 5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide
 
MOLECULAR BIOLOGY GRADE
 
PubChem Chemical ID: 6254
 
Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
 
Store at 2-8°C and protect from light and humidity.

 


Features

  • Highly stable and water soluble
  • Quick and simple assay
  • CAS#: 69-78-3
  • Molecular Formula:  C14H8N2O8S2
  • Molecular Weight: 396.36 g/mol 

 


Applications

  • Quantification of free sulfhydryl residues

 

 

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Protocol
BC87
Material Safety Data Sheet
BC87
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RC-483
English_US
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Dutch
English_UK
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RC-484
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Technical Literature
Protein Cross-Linking Handbook  A detailed guide to protein cross-linkers and their reactions.


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  • Xu, H. et al (2017) Green and Sustainable Technology for High-Efficiency and Low-Damage Manipulation of Densely Crosslinked Proteins. ACS Omega. DOI: 10.1021/acsomega.7b00154
  • Song, K. et al (2017) Non-toxic and clean crosslinking system for protein materials: Effect of extenders on crosslinking performance. Jclepro. 150:214
  • Garapaty, A. and Champion, J. (2016) Tunable particles alter macrophage uptake based oncombinatorial effects of physical properties. Bioengineering and Translational Medicine doi: 10.1002/btm2.10047
  • Song, K. et al (2016) Effects of chemical structures of polycarboxylic acids on molecular and performance manipulation of hair keratin.RSC Adv.DOI: 10.1039/C6RA08797C
  • Marlatt, V. L. et al (2016) Application of molecular endpoints in early life stage salmonid environmental biomonitoring. Aquatic Toxicology. 173:178
  • Bearat, H.H. et al (2012) Acta Biomaterialia. 8:3629
    • Catalog Description Size Price(USD) Qty
    BC87
    		 Ellman’s Reagent 5g
    $98.00
    RC-483
    		 Ellman's Reagent, 5-5-Dithiobis(2-nitrobenzoic-acid) 100 G
    $1,229.00
    RC-484
    		 Ellman's Reagent, 5-5-Dithiobis(2-nitrobenzoic-acid) 25 G
    $215.00