End-Repair dA-tailing Mix
End-Repair dA-tailing Mix
End-Repair dA-tailing Mix
| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2279 | End-Repair dA-tailing Mix | 24 reactions | $350.00 |
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Description
The End-Repair dA-tailing Mix converts damaged or incompatible 5′/3′ ends to 3' A tailed and 5′-phosphorylated DNA. This uses T4 DNA Polymerase’s 5′→3′ polymerase and 3′→5′ exonuclease actions to blunt the fragment ends before Taq DNA polymerase adds an A Tail at the 3' end and T4 Polynucleotide Kinase ensures 5′ phosphorylation.
- Reviews
The End-Repair dA-tailing Mix converts damaged or incompatible 5′/3′ ends to 3' A tailed and 5′-phosphorylated DNA. This uses T4 DNA Polymerase’s 5′→3′ polymerase and 3′→5′ exonuclease actions to blunt the fragment ends before Taq DNA polymerase adds an A Tail at the 3' end and T4 Polynucleotide Kinase ensures 5′ phosphorylation.