| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-790 | Immobilized Papain | 5ml Resin | $278.00 | |
| 786-812 | Immobilized Papain | 25ml resin | $1,362.00 |
Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
Click here to request bulk or custom sizes.
Features
Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge region. This cleavage results in the generation of three ~50kDa fragments; two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis.
The Fab and Fc fragments can be further separated with either Immobilized Protein A or ion exchange chromatography.
Immobilized Papain is a convenient reagent for producing Fab and Fc fragments as it avoids the need to remove the papain enzyme after digestion.
Click here to request bulk or custom sizes.
Features
