Luciferase (Firefly, Recombinant)

Firefly luciferase is a unique photoprotein used in bioluminescence for high sensitive detection and quantification of ATP and as a reporter for studying gene function and regulation.  Luciferase (Firefly, Recombinant) is recombinant 62 kDa protein expressed in E. Coli.  

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Highlights

• Single band purity, >99 % by SDS-PAGE
• High specific activity: ≥1 x 10E10 units/mg   

Features

• Molecular weight: 62 kDa
• Purity: >99 % by SDS-PAGE. Single band purity
• Protein amount: determined based on absorbance at 280 nm and molecular extinction co-efficeint of Luciferase
• Source: E. coli
• Shipping and handling: Luciferase (Firefly, Recombinant) is shipped on dry ice. Store at -20°C. Avoid repeated thawing and thaw on ice. When stored properly as advised the enzyme is stable for 1 year. For long term storage make small aliquotes and store at -70°C. For making enzyme dilutions it is recomended to add 1 % carrier protein such as BSA to the dilution buffer.

Application(s)

• Firefly luciferase is used in bioluminescence for high sensitive detection and quantification of ATP
• Firefly luciferase is used as reporter for studying gene function and regulation.