| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2299 | T7 Endonuclease I | 1,000U | $225.00 |
T7 Endonuclease I recognizes and cleaves mis-matched DNA. The cleavage takes place at the first, second or third phosphodiester bond that is 5´ to the mismatch. Mutations generated by CRISPR/ZFN/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. T7 Endonuclease I can recognize and cleave mismatched DNA length more than 1bp. It is used to estimate the mutation frequency of genone edited cells.
Product Source
E coli cells expressing T7 Endonuclease I gene.
Product Includes
10X T7 Endonuclease I reaction buffer composition
100 mM Tris-HCL, 500 mM KCl,100 mM MgCl2, 10 mM DTT, pH 7.5 @ 25ºC
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature
–20ºC
Quality Control assays
T7 Endonuclease I is free from detectable contaminating nuclease activities.
T7 Endonuclease I recognizes and cleaves mis-matched DNA. The cleavage takes place at the first, second or third phosphodiester bond that is 5´ to the mismatch. Mutations generated by CRISPR/ZFN/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. T7 Endonuclease I can recognize and cleave mismatched DNA length more than 1bp. It is used to estimate the mutation frequency of genone edited cells.
Product Source
E coli cells expressing T7 Endonuclease I gene.
Product Includes
10X T7 Endonuclease I reaction buffer composition
100 mM Tris-HCL, 500 mM KCl,100 mM MgCl2, 10 mM DTT, pH 7.5 @ 25ºC
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC
Storage Temperature
–20ºC
Quality Control assays
T7 Endonuclease I is free from detectable contaminating nuclease activities.
