Evans Blue

Evans blue is a synthetic bis-azo dye. It is used in histology and fluorescent microscopy. It has been used to check cell viability. It is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue.

Specifications:

Synonyms:                                       C.I. 23860; Direct Blue 53

CAS No:                                            314-13-6

Chemical Formula:                          C34H24N6Na4O14S4

Formula Weight:                              960.82

Description:                                      Dark brown to black to brownish-red to purple crystalline powder

Loss on drying (110°C/1hr):            < 10%

Solubility (0.1% H2O):                     Clear blue solution

Assay on absorptivity bases:          >85%

Absorptivity 1%/1cm

At Lambda Max 608 nm:                >650