FOCUS™ Membrane Proteins (3 Citations)
FOCUS™ Membrane Proteins is a rapid and highly reproducible method for preparation of membrane or hydrophobic proteins from biological samples for 2D-gel analysis or other applications. Membrane proteins are extracted with a single step phase partition, with an efficiency greater than 90% with minimal cross-contamination from hydrophilic proteins.
The kit is provided with reagents necessary for extraction of membrane proteins and their subsequent preparation for isoelectric focusing or 2D gel resolution for improved spot resolution.
FOCUS™ Membrane Proteins kit is designed for >50 preps, where 1 prep is:
- 100mg mammalian tissue
50µl wet animal cell pellet
50µl wet yeast pellet
50µl wet bacteria pellet
250mg plant tissue
Phase partition based extraction of membrane proteins.
Fractions suitable for 2D electrophoresis and other applications.
Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other sources.
Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.
|Material Safety Data Sheet|
|Mass Spectrometry Sample Prep Handbook||A guide to the preparation of protein samples for Mass Spectrometry, including protein extraction, clean-up and peptide generation.|
|Plant Proteomics Handbook|
|Protein Electrophoresis Handbook||A guide to 1D and 2D protein electrophoresis products, including protein markers, electrophoresis buffers, 2D electrophoresis reagents, clean-up reagents and stains. The guide also offers protein sample preparation products.|
|Sample Preparation Handbook||For Lysis Buffers, Fractionation, Dialysis, Protein Concentration and Enrichment|