FOCUS™ Signal Proteins

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in lipid rafts. Lipid raft localized proteins have been shown to be involved in intracellular membrane trafficking and signaling.

• 100mg mammalian tissue
• 50µl wet animal cell pellet
• 50µl wet yeast pellet
• 50µl wet bacteria pellet
• 250mg plant tissue

Features

•  Fractionate lipid raft localized proteins.
•  A 2D electrophoresis compatible buffer is provided.

Applications

•  Isolate signal proteins that are localized to lipid rafts.
•  Study movement of activated proteins to and from lipid rafts.
•  Suitable for 2D gel electrophoresis, proteomics and cell signaling studies.