G-Fusion Cloning Kit
| Catalog | Description | Size | Price (USD) | Qty |
|---|---|---|---|---|
| 786-2199 | G-Fusion Cloning Kit | 10 reactions | $185.00 | |
| 786-2200 | G-Fusion Cloning Kit | 50 reactions | $740.00 |
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Description
G-Fusion is a simple, rapid and highly efficient directional cloning of one or more fragments of DNA into any vector. The linearized vector can be generated by PCR or restriction enzymes. The PCR fragments can be generated with high fidelity DNA polymerase with primers having 15 to 18 bases of homology at their linear ends to where the product needs to fuse.
Items included:
- G-Fusion enzyme mix (5X)
- PCR master mix (2X)
- Competent cells.
Features:
- Any insert cloning at any site within any vector
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with > 95% positive clones
Storage Temperature:
- G-Fusion enzyme mix (5X) at -20ºC
- PCR master mix (2X) at -20ºC
- Competent cells at -80ºC
- Reviews
G-Fusion is a simple, rapid and highly efficient directional cloning of one or more fragments of DNA into any vector. The linearized vector can be generated by PCR or restriction enzymes. The PCR fragments can be generated with high fidelity DNA polymerase with primers having 15 to 18 bases of homology at their linear ends to where the product needs to fuse.
Items included:
- G-Fusion enzyme mix (5X)
- PCR master mix (2X)
- Competent cells.
Features:
- Any insert cloning at any site within any vector
- Joining multiple large fragments at once
- Precise insertion at a desired orientation
- Rapid and high efficiency with > 95% positive clones
Storage Temperature:
- G-Fusion enzyme mix (5X) at -20ºC
- PCR master mix (2X) at -20ºC
- Competent cells at -80ºC